Abstract
Cells with a genetic defect affecting a biological activity and/or a cell phenotype are generally called "cell mutants" and are a highly useful tool in genetic, biochemical, as well as cell biological research. To investigate peroxisome biogenesis and human peroxisome biogenesis disorders, more than a dozen complementation groups of Chinese hamster ovary (CHO) cell mutants defective in peroxisome assembly have been successfully isolated and established as a model system. Moreover, successful PEX gene cloning studies by taking advantage of rapid functional complementation assay of CHO cell mutants invaluably contributed to the accomplishment of isolation of pathogenic genes responsible for peroxisome biogenesis diseases. Molecular mechanisms of peroxisome assembly are currently investigated by making use of such mammalian cell mutants.
| Original language | English |
|---|---|
| Pages (from-to) | 1374-1381 |
| Number of pages | 8 |
| Journal | Biochimica et Biophysica Acta - Molecular Cell Research |
| Volume | 1763 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 1 2006 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology
Cite this
Lessons from peroxisome-deficient Chinese hamster ovary (CHO) cell mutants. / Fujiki, Yukio; Okumoto, Kanji; Kinoshita, Naohiko; Ghaedi, Kamran.
In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1763, No. 12, 01.12.2006, p. 1374-1381.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Lessons from peroxisome-deficient Chinese hamster ovary (CHO) cell mutants
AU - Fujiki, Yukio
AU - Okumoto, Kanji
AU - Kinoshita, Naohiko
AU - Ghaedi, Kamran
PY - 2006/12/1
Y1 - 2006/12/1
N2 - Cells with a genetic defect affecting a biological activity and/or a cell phenotype are generally called "cell mutants" and are a highly useful tool in genetic, biochemical, as well as cell biological research. To investigate peroxisome biogenesis and human peroxisome biogenesis disorders, more than a dozen complementation groups of Chinese hamster ovary (CHO) cell mutants defective in peroxisome assembly have been successfully isolated and established as a model system. Moreover, successful PEX gene cloning studies by taking advantage of rapid functional complementation assay of CHO cell mutants invaluably contributed to the accomplishment of isolation of pathogenic genes responsible for peroxisome biogenesis diseases. Molecular mechanisms of peroxisome assembly are currently investigated by making use of such mammalian cell mutants.
AB - Cells with a genetic defect affecting a biological activity and/or a cell phenotype are generally called "cell mutants" and are a highly useful tool in genetic, biochemical, as well as cell biological research. To investigate peroxisome biogenesis and human peroxisome biogenesis disorders, more than a dozen complementation groups of Chinese hamster ovary (CHO) cell mutants defective in peroxisome assembly have been successfully isolated and established as a model system. Moreover, successful PEX gene cloning studies by taking advantage of rapid functional complementation assay of CHO cell mutants invaluably contributed to the accomplishment of isolation of pathogenic genes responsible for peroxisome biogenesis diseases. Molecular mechanisms of peroxisome assembly are currently investigated by making use of such mammalian cell mutants.
UR - http://www.scopus.com/inward/record.url?scp=33845358982&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845358982&partnerID=8YFLogxK
U2 - 10.1016/j.bbamcr.2006.09.012
DO - 10.1016/j.bbamcr.2006.09.012
M3 - Review article
C2 - 17045664
AN - SCOPUS:33845358982
VL - 1763
SP - 1374
EP - 1381
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
SN - 0167-4889
IS - 12
ER -