Letter: Correlation between phosphorylation ratios by matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis and enzyme kinetics

Jeong Hun Kang, Masanori Kuramoto, Akira Tsuchiya, Riki Toita, Daisuke Asai, Yuko T. Sato, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

To identify the correlation between the phosphorylation ratios by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-ToF MS) analysis and enzyme kinetics (Km, Vmax and Vmax/Km) it is important to understand whether MALDI-ToF MS can be applied for monitoring the properties of peptides that are substrates of protein kinases. The correlation between phosphorylation ratios and enzyme kinetics was examined using peptides for protein kinase C (PKC) and for 60 kDa phosphoprotein, encoded by the cellular sarcoma gene (c-Src). Phosphorylation ratios, analyzed by MALDI-ToF MS, showed a higher correlation coefficient (r=>+0.7) for Vmax/Km compared with that (r= < ±0.6) for Km or Vmax. For ion modes, a higher correlation coefficient between phosphorylation ratios and Vmax/ Km was identified in the positive mode (r=> + 0.7) compared with that in the negative mode (r=<+0.5). These results suggest that MALDI-ToF MS is a useful tool to evaluate Vmax/Kmax of peptides for protein kinases.

Original languageEnglish
Pages (from-to)261-265
Number of pages5
JournalEuropean Journal of Mass Spectrometry
Volume14
Issue number4
DOIs
Publication statusPublished - Oct 15 2008

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Enzyme kinetics
phosphorylation
Phosphorylation
Ionization
Mass spectrometry
enzymes
Desorption
mass spectroscopy
desorption
peptides
ionization
Lasers
kinetics
matrices
proteins
Protein Kinases
Peptides
lasers
Phosphoproteins
correlation coefficients

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Spectroscopy

Cite this

Letter : Correlation between phosphorylation ratios by matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis and enzyme kinetics. / Kang, Jeong Hun; Kuramoto, Masanori; Tsuchiya, Akira; Toita, Riki; Asai, Daisuke; Sato, Yuko T.; Mori, Takeshi; Niidome, Takuro; Katayama, Yoshiki.

In: European Journal of Mass Spectrometry, Vol. 14, No. 4, 15.10.2008, p. 261-265.

Research output: Contribution to journalArticle

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