Levels of the EMT-related protein Snail/Slug are not correlated with p53/p63 in cutaneous squamouscell carcinoma

Hongxiang Chen, Masakazu Takahara, Lining Xie, Satoshi Takeuchi, Yating Tu, Takeshi Nakahara, Hiroshi Uchi, Yoichi Moroi, Masutaka Furue

Research output: Contribution to journalArticle

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Abstract

Background The contribution of the E-cadherin transcriptional repressors Snail and Slug to invasion and metastasis has strengthened the evidence for the importance of epithelial-mesenchymal transition (EMT) in carcinoma progression. However, to the best of our knowledge, no study has described the immunohistochemical staining of the EMT-related proteins Snail/Slug in skin tumors and the correlation between Snail/Slug and tumor suppressor p53/p63. Methods We performed immunohistological staining of Snail, Slug, E-cadherin, p53 and p63 in 20 archived specimens each of seborrheic keratosis (SK), actinic keratosis (AK) and squamous cell carcinoma in situ (SCCIS), and 53 specimens of cutaneous squamous cell carcinomas (SCC). Fifteen normal skin (NS) specimens served as controls. Results Significant negative correlations were observed between Snail and E-cadherin expression and between Slug and E-cadherin expression (Snail: R2 = 0.5432, p < 0.01; Slug: R2 = 0.4666, p < 0.01). Conclusions The staining intensities of Snail and Slug are associated with decreased E-cadherin staining in SCC and this may promote EMT. However, the staining intensities of p53 and p63 are not significantly correlated with the loss of E-cadherin.

Original languageEnglish
Pages (from-to)651-656
Number of pages6
JournalJournal of Cutaneous Pathology
Volume40
Issue number7
DOIs
Publication statusPublished - Jul 1 2013

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Gastropoda
Epithelial-Mesenchymal Transition
Cadherins
Carcinoma
Skin
Staining and Labeling
Proteins
Squamous Cell Carcinoma
Seborrheic Keratosis
Actinic Keratosis
Carcinoma in Situ
Neoplasms
Neoplasm Metastasis

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Dermatology

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Levels of the EMT-related protein Snail/Slug are not correlated with p53/p63 in cutaneous squamouscell carcinoma. / Chen, Hongxiang; Takahara, Masakazu; Xie, Lining; Takeuchi, Satoshi; Tu, Yating; Nakahara, Takeshi; Uchi, Hiroshi; Moroi, Yoichi; Furue, Masutaka.

In: Journal of Cutaneous Pathology, Vol. 40, No. 7, 01.07.2013, p. 651-656.

Research output: Contribution to journalArticle

Chen, Hongxiang ; Takahara, Masakazu ; Xie, Lining ; Takeuchi, Satoshi ; Tu, Yating ; Nakahara, Takeshi ; Uchi, Hiroshi ; Moroi, Yoichi ; Furue, Masutaka. / Levels of the EMT-related protein Snail/Slug are not correlated with p53/p63 in cutaneous squamouscell carcinoma. In: Journal of Cutaneous Pathology. 2013 ; Vol. 40, No. 7. pp. 651-656.
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T1 - Levels of the EMT-related protein Snail/Slug are not correlated with p53/p63 in cutaneous squamouscell carcinoma

AU - Chen, Hongxiang

AU - Takahara, Masakazu

AU - Xie, Lining

AU - Takeuchi, Satoshi

AU - Tu, Yating

AU - Nakahara, Takeshi

AU - Uchi, Hiroshi

AU - Moroi, Yoichi

AU - Furue, Masutaka

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N2 - Background The contribution of the E-cadherin transcriptional repressors Snail and Slug to invasion and metastasis has strengthened the evidence for the importance of epithelial-mesenchymal transition (EMT) in carcinoma progression. However, to the best of our knowledge, no study has described the immunohistochemical staining of the EMT-related proteins Snail/Slug in skin tumors and the correlation between Snail/Slug and tumor suppressor p53/p63. Methods We performed immunohistological staining of Snail, Slug, E-cadherin, p53 and p63 in 20 archived specimens each of seborrheic keratosis (SK), actinic keratosis (AK) and squamous cell carcinoma in situ (SCCIS), and 53 specimens of cutaneous squamous cell carcinomas (SCC). Fifteen normal skin (NS) specimens served as controls. Results Significant negative correlations were observed between Snail and E-cadherin expression and between Slug and E-cadherin expression (Snail: R2 = 0.5432, p < 0.01; Slug: R2 = 0.4666, p < 0.01). Conclusions The staining intensities of Snail and Slug are associated with decreased E-cadherin staining in SCC and this may promote EMT. However, the staining intensities of p53 and p63 are not significantly correlated with the loss of E-cadherin.

AB - Background The contribution of the E-cadherin transcriptional repressors Snail and Slug to invasion and metastasis has strengthened the evidence for the importance of epithelial-mesenchymal transition (EMT) in carcinoma progression. However, to the best of our knowledge, no study has described the immunohistochemical staining of the EMT-related proteins Snail/Slug in skin tumors and the correlation between Snail/Slug and tumor suppressor p53/p63. Methods We performed immunohistological staining of Snail, Slug, E-cadherin, p53 and p63 in 20 archived specimens each of seborrheic keratosis (SK), actinic keratosis (AK) and squamous cell carcinoma in situ (SCCIS), and 53 specimens of cutaneous squamous cell carcinomas (SCC). Fifteen normal skin (NS) specimens served as controls. Results Significant negative correlations were observed between Snail and E-cadherin expression and between Slug and E-cadherin expression (Snail: R2 = 0.5432, p < 0.01; Slug: R2 = 0.4666, p < 0.01). Conclusions The staining intensities of Snail and Slug are associated with decreased E-cadherin staining in SCC and this may promote EMT. However, the staining intensities of p53 and p63 are not significantly correlated with the loss of E-cadherin.

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