LiaRS reporter assay: A simple tool to identify lipid II binding moieties in lantibiotic nukacin ISK-1

Khaled M. Elsayed, Mohammad R. Islam, Abdullah-Al-Mahin, Jun ichi Nagao, Takeshi Zendo, Kenji Sonomoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Binding to lipid II is an important step in the mode of action of most lantibiotics targeting the bacterial cell wall. We applied the Bacillus subtilis two-component system, LiaRS, that is known to respond to antibiotics interfering with lipid II cycle, in order to evaluate lipid II binding activity of known bacteriocins and also to identify lipid II binding moieties in lantibiotic nukacin ISK-1. Using this method, we confirmed that the methyllanthionine ring in nukacin ISK-1 is crucial for lipid II binding as previously indicated. In this study, we further identified that the three N-terminal lysine residues (K1, K2, and K3) and the glycine (G5) residue in nukacin ISK-1 are also important in lipid II binding.

Original languageEnglish
Pages (from-to)398-401
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume123
Issue number3
DOIs
Publication statusPublished - Mar 1 2017

Fingerprint

Bacteriocins
Lipids
Assays
Antibiotics
Bacilli
Bacillus subtilis
Glycine
Cell Wall
Lysine
nukacin ISK-1
muramyl-NAc-(pentapeptide)pyrophosphoryl-undecaprenol
Amino acids
Cells
Anti-Bacterial Agents

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

LiaRS reporter assay : A simple tool to identify lipid II binding moieties in lantibiotic nukacin ISK-1. / Elsayed, Khaled M.; Islam, Mohammad R.; Abdullah-Al-Mahin; Nagao, Jun ichi; Zendo, Takeshi; Sonomoto, Kenji.

In: Journal of Bioscience and Bioengineering, Vol. 123, No. 3, 01.03.2017, p. 398-401.

Research output: Contribution to journalArticle

Elsayed, Khaled M. ; Islam, Mohammad R. ; Abdullah-Al-Mahin ; Nagao, Jun ichi ; Zendo, Takeshi ; Sonomoto, Kenji. / LiaRS reporter assay : A simple tool to identify lipid II binding moieties in lantibiotic nukacin ISK-1. In: Journal of Bioscience and Bioengineering. 2017 ; Vol. 123, No. 3. pp. 398-401.
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