Ligand-Induced Conformational Changes near the Active Site Regulating Enzyme Activity of Momorcharins from Seeds of Bitter Gourd

Chie Matsunaga, Yuuki Okada, Etsuko Nishimoto

Research output: Contribution to journalArticle

Abstract

It is reasonable to consider that Type I-ribosomal inactivation proteins (RIP) retain some specific affinity to harmful pathogens to complete the role as a bio-defense relating protein. In the present studies, it was shown that two Type I-RIPs, α- and β-momorcharins, maintained the abilities to bind with N-acetylglucosamine (NAG) to change the conformation around the active sites and to regulate their N-glycosidase activities. By the binding of NAG, the freedom of internal motion of Trp192 in α-momorcharin was increased 1.5 times near the active site and, on the other hand, the corresponding motion of Trp190 was limited 50% in β-momorcharin. The results in the fluorescence resonance excitation energy transfer experiments demonstrated that Trp-190 of β-momorcharin was kept away from Tyr-70 but Trp192 contrarily approached closer to the nearest neighboring Tyr residue consisting of the active center of α-momorcharin by the binding with NAG. These conformational changes near the active site close correlated with promotion and/or suppression of the N-glucosidase activities of β- and α-momorcharins.

Original languageEnglish
Pages (from-to)231-240
Number of pages10
JournalJournal of Fluorescence
Volume29
Issue number1
DOIs
Publication statusPublished - Jan 15 2019

Fingerprint

Momordica charantia
Enzyme activity
Seed
Catalytic Domain
Seeds
Ligands
Acetylglucosamine
Enzymes
suppression
promotion
energy
experiment
ability
Glucosidases
Fluorescence Resonance Energy Transfer
Excitation energy
Ribosomal Proteins
Glycoside Hydrolases
Pathogens
Energy transfer

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Psychology
  • Social Sciences (miscellaneous)
  • Sociology and Political Science
  • Spectroscopy
  • Clinical Biochemistry
  • Law

Cite this

Ligand-Induced Conformational Changes near the Active Site Regulating Enzyme Activity of Momorcharins from Seeds of Bitter Gourd. / Matsunaga, Chie; Okada, Yuuki; Nishimoto, Etsuko.

In: Journal of Fluorescence, Vol. 29, No. 1, 15.01.2019, p. 231-240.

Research output: Contribution to journalArticle

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