Ligand-Mediated Coating of Liposomes with Human Serum Albumin

Hikari Sato, Elnaz Nakhaei, Takahito Kawano, Masaharu Murata, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Coating liposome surfaces with human serum albumin (HSA) can improve the colloidal stability and prevent opsonization. HSA coating via specific binding with alkyl ligands is promising because although the ligand-mediated coating is relatively stable it can spontaneously exchange with fresh HSA. However, to achieve surface coating with HSA, multiple hydrophobic ligands must be exposed to an aqueous medium prior to binding with HSA. This presents a challenge, as hydrophobic ligands tend to be buried in the liposomal membrane. Here we present the first HSA modification of liposome surfaces via alkyl ligands. We found that a relatively short alkyl ligand, or a long alkyl ligand with a terminal carboxylate, could be exposed on the liposome surface without causing aggregation of the liposomes and these ligands could subsequently bind HSA. The resulting HSA-coated liposomes were as inert as conventional PEGylated liposomes in terms of macrophage recognition.

Original languageEnglish
Pages (from-to)2324-2331
Number of pages8
JournalLangmuir
Volume34
Issue number6
DOIs
Publication statusPublished - Feb 13 2018

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Fingerprint Dive into the research topics of 'Ligand-Mediated Coating of Liposomes with Human Serum Albumin'. Together they form a unique fingerprint.

Cite this