We have constructed the fusion proteins which contain multiple different specific binding sites and self-assemble to an ordered structure by their mutual specific interactions (termed NanoLEGO). Subunit of tetramer protein, super oxide reductase (SOR), was fused with PDZ protein, tax-interacting protein-1(TIP-1), and its recognition peptides(PDZ-pep). The genetically-modified subunits of SOR with TIP-1 and PDZ-pep could self-assemble and reconstruct the chimera tetramer with four binding sites. To investigate the self-assembling ability of SOR NanoLEGO, the interaction forces between TIP-1 and PDZ-pep were characterized by dynamic force spectroscopy, and the assembling property and structures were analyzed with turbidity measurement, phase contrast and fluorescence microscopy, and transmission electron microscopy. SOR NanoLEGOs were found to self-assemble and form chain-like constructs.
|Number of pages||2|
|Publication status||Published - Dec 1 2006|
|Event||55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan|
Duration: Sep 20 2006 → Sep 22 2006
|Other||55th Society of Polymer Science Japan Symposium on Macromolecules|
|Period||9/20/06 → 9/22/06|
All Science Journal Classification (ASJC) codes