Ligand screening of olfactory receptors

Kazushige Touhara, Sayako Katada, Takao Nakagawa, Yuki Oka

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

Olfactory receptors (ORs), which comprise the largest G protein-coupled receptor (GPCR) family, play a pivotal role in recognizing a variety of odorants in the vertebrate olfactory system. 1-4 Genome-wide repertoires of the OR family have become available in a variety of vertebrate species, such as human (~350 intact ORs) 5-7 and mouse (1300 to 1500 intact ORs), 8,9 as well as in invertebrates, including fruit fly (62 ORs), 10-12 nematode (~500 chemosensory receptors), 13,14 and mosquito (79 ORs) 15 (0.5 to 4% of the predicted proteomes). The vertebrate OR sequences can be separated into two classes: the Class I family, which was first found in fish and was therefore predicted to recognize water-soluble odorant molecules, such as amino acids and carboxylic acids; and the Class II family, which was not found in fish, and was therefore thought to have appeared during the transition from an aquatic to a terrestrial lifestyle. 16,17 Conserved motifs of five to 10 amino acids have been found that can identify ORs and distinguish them from other GPCRs. In addition, variable regions are found within transmembrane domains of ORs. As in other GPCRs, these might function as the ligand-binding site. 18-20 Across the OR family, the amino acid similarity is 37% on average, with an identity ranging from less than 20% to over 90%. 4.

Original languageEnglish
Title of host publicationG Protein-Coupled Receptors
Subtitle of host publicationStructure, Function, and Ligand Screening
PublisherCRC Press
Pages85-109
Number of pages25
ISBN (Electronic)9781420038279
ISBN (Print)0849327717, 9780849327711
Publication statusPublished - Jan 1 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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  • Cite this

    Touhara, K., Katada, S., Nakagawa, T., & Oka, Y. (2005). Ligand screening of olfactory receptors. In G Protein-Coupled Receptors: Structure, Function, and Ligand Screening (pp. 85-109). CRC Press.