Light chain shifting: Identification of a human plasma cell line actively undergoing light chain replacement

Hirofumi Tachibana, Hirotaka Haruta, Koji Yamada

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We identified an antibody-secreting human B-cell line (HTD8), which actively replaces the production of the original X light chain with a new λ chain (light chain shifting) at a high rate. Loss of the original rearranged λ light chain occurs by significantly reducing the amount of transcript expressed. Expression of the new λ chain, which replaces the original λ chain, occurs by rearranging new VJ segments on a previously excluded allele. V λ gene usage of these new rearrangements are biased toward V(λ)4, V(λ)6, and V(λ)10 families, which are known to be the least frequently used. In striking contrast to the plasma cell phenotype, recombination activating genes, RAG-1 and RAG-2, were expressed in the HTD8 cells and were shown to be necessary, but insufficient for inducing expression of the new λ chain. These results suggest that human plasma cells have the potential to actively undergo light chain replacement.

Original languageEnglish
Pages (from-to)198-207
Number of pages10
JournalBlood
Volume93
Issue number1
DOIs
Publication statusPublished - Jan 1 1999

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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