Light-triggered selective nitration of PsbO1 in isolated Arabidopsis thylakoid membranes is inhibited by photosynthetic electron transport inhibitors

Misa Takahashi, Jun Shigeto, Atsushi Sakamoto, Hiromichi Morikawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

PsbO1 is exclusively nitrated when isolated thylakoid membranes are incubated in a buffer bubbled with nitrogen dioxide (NO2) containing NO2 and nitrite. NO2 is the primary intermediate for this selective nitration. Isolated thylakoid membranes were incubated in NO2-bubbled buffer at 25°C in the light or dark. Protein analysis confirmed the selective nitration of PsbO1. Illumination was found to be essential in PsbO1 nitration. A nitration mechanism whereby nitratable tyrosine residues of PsbO1 are, prior to nitration, selectively photo-oxidized by photosynthetic electron transport to tyrosyl radicals to combine with NO2 to form 3-nitrotyrosine was hypothesized. We tested the electron transport inhibitors 3-(3,4-dichlorophenyl)-1,1- dimethylurea, sodium azide, and 1,5-diphenylcarbazide and found distinct inhibition of nitration of PsbO1. We also propose a possible nitration mechanism.

Original languageEnglish
Article numbere1263413
JournalPlant Signaling and Behavior
Volume11
Issue number12
DOIs
Publication statusPublished - Dec 1 2016

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nitrogen dioxide
thylakoids
electron transfer
Arabidopsis
buffers
sodium azide
nitrites
tyrosine
lighting
proteins

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Light-triggered selective nitration of PsbO1 in isolated Arabidopsis thylakoid membranes is inhibited by photosynthetic electron transport inhibitors. / Takahashi, Misa; Shigeto, Jun; Sakamoto, Atsushi; Morikawa, Hiromichi.

In: Plant Signaling and Behavior, Vol. 11, No. 12, e1263413, 01.12.2016.

Research output: Contribution to journalArticle

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