TY - JOUR
T1 - Light-triggered selective nitration of PsbO1 in isolated Arabidopsis thylakoid membranes is inhibited by photosynthetic electron transport inhibitors
AU - Takahashi, Misa
AU - Shigeto, Jun
AU - Sakamoto, Atsushi
AU - Morikawa, Hiromichi
N1 - Funding Information:
We thank Professors Junichi Mano of Yamaguchi University and Tsuyoshi Endo of Kyoto University for their interest in this work and for invaluable discussions during the course of this study. This work was supported by a grant from the Nippon Life Insurance Foundation (to MT), a grant from the Nissan Science Foundation (to MT), Grant-in-Aid for Creative Scientific Research from the Japan Science and Technology Agency no.13GS0023 (to HM), and Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science no. 15710149 (to MT).
Publisher Copyright:
© 2016 Taylor & Francis Group, LLC.
PY - 2016/12/1
Y1 - 2016/12/1
N2 - PsbO1 is exclusively nitrated when isolated thylakoid membranes are incubated in a buffer bubbled with nitrogen dioxide (NO2) containing NO2 and nitrite. NO2 is the primary intermediate for this selective nitration. Isolated thylakoid membranes were incubated in NO2-bubbled buffer at 25°C in the light or dark. Protein analysis confirmed the selective nitration of PsbO1. Illumination was found to be essential in PsbO1 nitration. A nitration mechanism whereby nitratable tyrosine residues of PsbO1 are, prior to nitration, selectively photo-oxidized by photosynthetic electron transport to tyrosyl radicals to combine with NO2 to form 3-nitrotyrosine was hypothesized. We tested the electron transport inhibitors 3-(3,4-dichlorophenyl)-1,1- dimethylurea, sodium azide, and 1,5-diphenylcarbazide and found distinct inhibition of nitration of PsbO1. We also propose a possible nitration mechanism.
AB - PsbO1 is exclusively nitrated when isolated thylakoid membranes are incubated in a buffer bubbled with nitrogen dioxide (NO2) containing NO2 and nitrite. NO2 is the primary intermediate for this selective nitration. Isolated thylakoid membranes were incubated in NO2-bubbled buffer at 25°C in the light or dark. Protein analysis confirmed the selective nitration of PsbO1. Illumination was found to be essential in PsbO1 nitration. A nitration mechanism whereby nitratable tyrosine residues of PsbO1 are, prior to nitration, selectively photo-oxidized by photosynthetic electron transport to tyrosyl radicals to combine with NO2 to form 3-nitrotyrosine was hypothesized. We tested the electron transport inhibitors 3-(3,4-dichlorophenyl)-1,1- dimethylurea, sodium azide, and 1,5-diphenylcarbazide and found distinct inhibition of nitration of PsbO1. We also propose a possible nitration mechanism.
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U2 - 10.1080/15592324.2016.1263413
DO - 10.1080/15592324.2016.1263413
M3 - Article
C2 - 27901641
AN - SCOPUS:85008195324
VL - 11
JO - Plant Signaling and Behavior
JF - Plant Signaling and Behavior
SN - 1559-2316
IS - 12
M1 - e1263413
ER -