Lignin dehydrogenative polymerization mechanism: A poplar cell wall peroxidase directly oxidizes polymer lignin and produces in vitro dehydrogenative polymer rich in β-O-4 linkage

Shinya Sasaki, Tomoaki Nishida, Yuji Tsutsumi, Ryuichiro Kondo

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

An investigation was performed to determine whether lignin dehydrogenative polymerization proceeds via radical mediation or direct oxidation by peroxidases. It was found that coniferyl alcohol radical transferred quickly to sinapyl alcohol. The transfer to syringaresinol was slower, however, the transfer to polymeric lignols occurred very slightly. This result suggests that the radical mediator theory does not sufficiently explain the mechanism for dehydrogenative polymerization of lignin. A cationic cell wall peroxidase (CWPO-C) from poplar (Populus alba L.) callus showed a strong substrate preference for sinapyl alcohol and the sinapyl alcohol dimer, syringaresinol. Moreover, CWPO-C was capable of oxidizing high-molecular-weight sinapyl alcohol polymers and ferrocytochrome c. Therefore, the CWPO-C characteristics are important to produce polymer lignin. The results suggest that CWPO-C may be a peroxidase isoenzyme responsible for the lignification of plant cell walls.

Original languageEnglish
Pages (from-to)197-201
Number of pages5
JournalFEBS Letters
Volume562
Issue number1-3
DOIs
Publication statusPublished - Mar 26 2004

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Lignin
Polymerization
Cell Wall
Peroxidase
Polymers
Cells
Populus
Peroxidases
Bony Callus
Plant Cells
Cytochromes c
Dimers
Isoenzymes
Molecular Weight
Molecular weight
Oxidation
sinapyl alcohol
In Vitro Techniques
Substrates
syringaresinol

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Lignin dehydrogenative polymerization mechanism : A poplar cell wall peroxidase directly oxidizes polymer lignin and produces in vitro dehydrogenative polymer rich in β-O-4 linkage. / Sasaki, Shinya; Nishida, Tomoaki; Tsutsumi, Yuji; Kondo, Ryuichiro.

In: FEBS Letters, Vol. 562, No. 1-3, 26.03.2004, p. 197-201.

Research output: Contribution to journalArticle

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