TY - JOUR
T1 - Lignin deposits in pedicel xylem vessels regulate water transport during seed maturation in soybean
AU - Itoyama, Haruka
AU - Nakagawa, Andressa C.S.
AU - Ariyoshi, Yuri
AU - Ario, Nobuyuki
AU - Yuasa, Takashi
AU - Iwaya-Inoue, Mari
AU - Ishibashi, Yushi
N1 - Funding Information:
This work was supported by Japan Society for the Promotion of Science (Grant no. JP16H04867).
Funding Information:
This work was supported by Japan Society for the Promotion of Science (Grant no. JP16H04867).
Publisher Copyright:
© 2020 The Authors. Crop Science © 2020 Crop Science Society of America
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Seed desiccation during maturation is important for seed post-maturation behavior and harvest. However, the desiccation mechanism in soybean [Glycine max (L.) Merr.] seed maturation is hardly known. In this study, we found that water content in seeds, pods, and pedicels decreased faster than that in peduncles and stems. Therefore, we focused on the role of the pedicel during seed maturation. Morphological analysis revealed deposits in xylem vessels in the pedicel at 61 d after flowering (DAF), but no deposits in the peduncle. Microarray analysis showed that lignin biosynthesis-related genes were expressed in the pedicel at 61 DAF. Among them, the expression of GmPAL, Gm4CL, GmC3H, and GmCAD increased in the pedicel during seed maturation. Pedicel lignin content also increased by 61 DAF; lignin accumulated in xylem vessels. These results suggest that lignin deposits in pedicel xylem vessels cause soybean seed desiccation during maturation.
AB - Seed desiccation during maturation is important for seed post-maturation behavior and harvest. However, the desiccation mechanism in soybean [Glycine max (L.) Merr.] seed maturation is hardly known. In this study, we found that water content in seeds, pods, and pedicels decreased faster than that in peduncles and stems. Therefore, we focused on the role of the pedicel during seed maturation. Morphological analysis revealed deposits in xylem vessels in the pedicel at 61 d after flowering (DAF), but no deposits in the peduncle. Microarray analysis showed that lignin biosynthesis-related genes were expressed in the pedicel at 61 DAF. Among them, the expression of GmPAL, Gm4CL, GmC3H, and GmCAD increased in the pedicel during seed maturation. Pedicel lignin content also increased by 61 DAF; lignin accumulated in xylem vessels. These results suggest that lignin deposits in pedicel xylem vessels cause soybean seed desiccation during maturation.
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U2 - 10.1002/csc2.20121
DO - 10.1002/csc2.20121
M3 - Article
AN - SCOPUS:85081731867
SN - 0011-183X
VL - 60
SP - 954
EP - 960
JO - Crop Science
JF - Crop Science
IS - 2
ER -