TY - JOUR
T1 - Limulus kexin
T2 - A new type of Kex2-like endoprotease specifically expressed in hemocytes of the horseshoe crab
AU - Kawabata, Shun Ichiro
AU - Saeki, Kazuko
AU - Iwanaga, Sadaaki
N1 - Funding Information:
Acknowledgements." We thank Miki Nakama for excellent technical assistance and Satomi Matsumura for secretarial assistance. This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.
PY - 1996/5/20
Y1 - 1996/5/20
N2 - A Kex2-like protease was identified in hemocytes of the horseshoe crab (Tachypleus tridentatus), named limulus kexin, and a full-length cDNA was obtained from a hemocyte cDNA library. The deduced amino acid sequence contains 752 residues, composed of five domains with a signal sequence, a propeptide, a catalytic domain, a Ser/Thr-rich domain, and a transmembrane domain. The domain organization is very similar to that of the yeast Kex2 except that limulus kexin does not have a cytoplasmic tail. The catalytic domain exhibits striking sequence identities with those of furins, especially Drosophila furin1 (79%). Northern blotting showed specific expression of limulus kexin in hemocytes, suggesting the involvement in proteolytic processing of the granule components of hemocytes.
AB - A Kex2-like protease was identified in hemocytes of the horseshoe crab (Tachypleus tridentatus), named limulus kexin, and a full-length cDNA was obtained from a hemocyte cDNA library. The deduced amino acid sequence contains 752 residues, composed of five domains with a signal sequence, a propeptide, a catalytic domain, a Ser/Thr-rich domain, and a transmembrane domain. The domain organization is very similar to that of the yeast Kex2 except that limulus kexin does not have a cytoplasmic tail. The catalytic domain exhibits striking sequence identities with those of furins, especially Drosophila furin1 (79%). Northern blotting showed specific expression of limulus kexin in hemocytes, suggesting the involvement in proteolytic processing of the granule components of hemocytes.
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U2 - 10.1016/0014-5793(96)00440-1
DO - 10.1016/0014-5793(96)00440-1
M3 - Article
C2 - 8647282
AN - SCOPUS:0029929981
VL - 386
SP - 201
EP - 204
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2-3
ER -