LINE-1 Hypomethylation Is Associated With Malignant Traits and Cell Proliferation in Lung Adenocarcinoma

Hirokazu Kitahara; Tatsuro Okamoto; Shinichiro Shimamatsu; Mikihiro Kohno; Yosuke Morodomi; Tetsuzo Tagawa; Hiroyuki Kitao; Shinji Okano; Yoshinao Oda; Yoshihiko Maehara; Masaki Mori

doi:10.21873/anticanres.14579

LINE-1 Hypomethylation Is Associated With Malignant Traits and Cell Proliferation in Lung Adenocarcinoma

Hirokazu Kitahara, Tatsuro Okamoto, Shinichiro Shimamatsu, Mikihiro Kohno, Yosuke Morodomi, Tetsuzo Tagawa, Hiroyuki Kitao, Shinji Okano, Yoshinao Oda, Yoshihiko Maehara, Masaki Mori

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND/AIM: Long interspersed nuclear element-1 (LINE-1) methylation status is a marker for global DNA methylation. However, the relationship between LINE-1 methylation and the biology of lung adenocarcinoma remains unclear. Here, we aimed to examine the role of LINE-1 in lung cancer.

MATERIALS AND METHODS: LINE-1 methylation levels were quantified by bisulfite pyrosequencing of resected tumor specimens from 162 patients with lung adenocarcinoma. The relationships of LINE-1 methylation with clinicopathological factors, gene mutations, and Ki-67 immunoreactivity were investigated.

RESULTS: LINE-1 hypomethylation was associated with tumor invasion and advanced stage. TP53 mutations were more frequently detected in the LINE-1 hypomethylation group than in the hypermethylation group. LINE-1 hypomethylation was associated with poor recurrence-free survival, high maximum standardized uptake value in positron-emission tomography, and high Ki-67 expression in tumors.

CONCLUSION: LINE-1 hypomethylation was associated with high-grade malignancy and poor prognosis in lung adenocarcinoma, but was not related to driver mutations.

Original language	English
Pages (from-to)	5659-5666
Number of pages	8
Journal	Anticancer research
Volume	40
Issue number	10
DOIs	https://doi.org/10.21873/anticanres.14579
Publication status	Published - Oct 2020

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@article{7ec513cc43fa4d6d867c5d5371c4d9ab,

title = "LINE-1 Hypomethylation Is Associated With Malignant Traits and Cell Proliferation in Lung Adenocarcinoma",

abstract = "BACKGROUND/AIM: Long interspersed nuclear element-1 (LINE-1) methylation status is a marker for global DNA methylation. However, the relationship between LINE-1 methylation and the biology of lung adenocarcinoma remains unclear. Here, we aimed to examine the role of LINE-1 in lung cancer.MATERIALS AND METHODS: LINE-1 methylation levels were quantified by bisulfite pyrosequencing of resected tumor specimens from 162 patients with lung adenocarcinoma. The relationships of LINE-1 methylation with clinicopathological factors, gene mutations, and Ki-67 immunoreactivity were investigated.RESULTS: LINE-1 hypomethylation was associated with tumor invasion and advanced stage. TP53 mutations were more frequently detected in the LINE-1 hypomethylation group than in the hypermethylation group. LINE-1 hypomethylation was associated with poor recurrence-free survival, high maximum standardized uptake value in positron-emission tomography, and high Ki-67 expression in tumors.CONCLUSION: LINE-1 hypomethylation was associated with high-grade malignancy and poor prognosis in lung adenocarcinoma, but was not related to driver mutations.",

author = "Hirokazu Kitahara and Tatsuro Okamoto and Shinichiro Shimamatsu and Mikihiro Kohno and Yosuke Morodomi and Tetsuzo Tagawa and Hiroyuki Kitao and Shinji Okano and Yoshinao Oda and Yoshihiko Maehara and Masaki Mori",

year = "2020",

month = oct,

doi = "10.21873/anticanres.14579",

language = "English",

volume = "40",

pages = "5659--5666",

journal = "Anticancer Research",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

number = "10",

}

TY - JOUR

T1 - LINE-1 Hypomethylation Is Associated With Malignant Traits and Cell Proliferation in Lung Adenocarcinoma

AU - Kitahara, Hirokazu

AU - Okamoto, Tatsuro

AU - Shimamatsu, Shinichiro

AU - Kohno, Mikihiro

AU - Morodomi, Yosuke

AU - Tagawa, Tetsuzo

AU - Kitao, Hiroyuki

AU - Okano, Shinji

AU - Oda, Yoshinao

AU - Maehara, Yoshihiko

AU - Mori, Masaki

PY - 2020/10

Y1 - 2020/10

N2 - BACKGROUND/AIM: Long interspersed nuclear element-1 (LINE-1) methylation status is a marker for global DNA methylation. However, the relationship between LINE-1 methylation and the biology of lung adenocarcinoma remains unclear. Here, we aimed to examine the role of LINE-1 in lung cancer.MATERIALS AND METHODS: LINE-1 methylation levels were quantified by bisulfite pyrosequencing of resected tumor specimens from 162 patients with lung adenocarcinoma. The relationships of LINE-1 methylation with clinicopathological factors, gene mutations, and Ki-67 immunoreactivity were investigated.RESULTS: LINE-1 hypomethylation was associated with tumor invasion and advanced stage. TP53 mutations were more frequently detected in the LINE-1 hypomethylation group than in the hypermethylation group. LINE-1 hypomethylation was associated with poor recurrence-free survival, high maximum standardized uptake value in positron-emission tomography, and high Ki-67 expression in tumors.CONCLUSION: LINE-1 hypomethylation was associated with high-grade malignancy and poor prognosis in lung adenocarcinoma, but was not related to driver mutations.

AB - BACKGROUND/AIM: Long interspersed nuclear element-1 (LINE-1) methylation status is a marker for global DNA methylation. However, the relationship between LINE-1 methylation and the biology of lung adenocarcinoma remains unclear. Here, we aimed to examine the role of LINE-1 in lung cancer.MATERIALS AND METHODS: LINE-1 methylation levels were quantified by bisulfite pyrosequencing of resected tumor specimens from 162 patients with lung adenocarcinoma. The relationships of LINE-1 methylation with clinicopathological factors, gene mutations, and Ki-67 immunoreactivity were investigated.RESULTS: LINE-1 hypomethylation was associated with tumor invasion and advanced stage. TP53 mutations were more frequently detected in the LINE-1 hypomethylation group than in the hypermethylation group. LINE-1 hypomethylation was associated with poor recurrence-free survival, high maximum standardized uptake value in positron-emission tomography, and high Ki-67 expression in tumors.CONCLUSION: LINE-1 hypomethylation was associated with high-grade malignancy and poor prognosis in lung adenocarcinoma, but was not related to driver mutations.

U2 - 10.21873/anticanres.14579

DO - 10.21873/anticanres.14579

M3 - Article

C2 - 32988890

SN - 0250-7005

VL - 40

SP - 5659

EP - 5666

JO - Anticancer Research

JF - Anticancer Research

IS - 10

ER -

LINE-1 Hypomethylation Is Associated With Malignant Traits and Cell Proliferation in Lung Adenocarcinoma

Abstract

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