LINE-1 Hypomethylation is associated with malignant traits and cell proliferation in lung adenocarcinoma

Hirokazu Kitahara, Tatsuro Okamoto, Shinichiro Shimamatsu, Mikihiro Kohno, Yosuke Morodomi, Tetsuzo Tagawa, Hiroyuki Kitao, Shinji Okano, Yoshinao Oda, Yoshihiko Maehara, Masaki Mori

Research output: Contribution to journalArticlepeer-review

Abstract

Background/Aim: Long interspersed nuclear element-1 (LINE-1) methylation status is a marker for global DNA methylation. However, the relationship between LINE-1 methylation and the biology of lung adenocarcinoma remains unclear. Here, we aimed to examine the role of LINE-1 in lung cancer. Materials and Methods: LINE-1 methylation levels were quantified by bisulfite pyrosequencing of resected tumor specimens from 162 patients with lung adenocarcinoma. The relationships of LINE-1 methylation with clinicopathological factors, gene mutations, and Ki-67 immunoreactivity were investigated. Results: LINE-1 hypomethylation was associated with tumor invasion and advanced stage. TP53 mutations were more frequently detected in the LINE-1 hypomethylation group than in the hypermethylation group. LINE-1 hypomethylation was associated with poor recurrence-free survival, high maximum standardized uptake value in positron-emission tomography, and high Ki-67 expression in tumors. Conclusion: LINE-1 hypomethylation was associated with high-grade malignancy and poor prognosis in lung adenocarcinoma, but was not related to driver mutations.

Original languageEnglish
Pages (from-to)5659-5666
Number of pages8
JournalAnticancer research
Volume40
Issue number10
DOIs
Publication statusPublished - Oct 2020

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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