Line-scanning microscopy for time-gated and spectrally resolved fluorescence imaging

Ryosuke Nakamura, Yoshihiro Izumi, Shin'Ichiro Kajiyama, Akio Kobayashi, Yasuo Kanematsu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Laser-scanning fluorescence microscopy for efficient acquisition of time-gated and spectrally resolved fluorescence images was developed based on line illumination of the laser beam and detection of the fluorescence image through a slit. In this optical arrangement, the fluorescence image was obtained by scanning only one axis perpendicular to the excitation line, and the acquisition time was significantly reduced compared with conventional laser-scanning confocal microscopy. A multidimensional fluorescence dataset consisting of fluorescence intensities as a function of x-position, y-position, fluorescence wavelength, and delay time after photoexcitation was analyzed and decomposed based on the parallel factor analysis model. The performance of the line-scanning microscopy was examined by applying it to the analysis of one of the plant defense responses, accumulation of antimicrobial compounds of phytoalexin in oat (Avena sativa), induced by the elicitor treatment.

Original languageEnglish
Pages (from-to)51-62
Number of pages12
JournalJournal of Biological Physics
Volume34
Issue number1-2 SPEC. ISS.
DOIs
Publication statusPublished - Apr 1 2008
Externally publishedYes

Fingerprint

Optical Imaging
Microscopy
Fluorescence
microscopy
fluorescence
scanning
Confocal Microscopy
acquisition
oats
factor analysis
Lighting
Fluorescence Microscopy
Statistical Factor Analysis
photoexcitation
Lasers
lasers
slits
time lag
illumination
laser beams

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Atomic and Molecular Physics, and Optics
  • Molecular Biology
  • Cell Biology

Cite this

Line-scanning microscopy for time-gated and spectrally resolved fluorescence imaging. / Nakamura, Ryosuke; Izumi, Yoshihiro; Kajiyama, Shin'Ichiro; Kobayashi, Akio; Kanematsu, Yasuo.

In: Journal of Biological Physics, Vol. 34, No. 1-2 SPEC. ISS., 01.04.2008, p. 51-62.

Research output: Contribution to journalArticle

Nakamura, Ryosuke ; Izumi, Yoshihiro ; Kajiyama, Shin'Ichiro ; Kobayashi, Akio ; Kanematsu, Yasuo. / Line-scanning microscopy for time-gated and spectrally resolved fluorescence imaging. In: Journal of Biological Physics. 2008 ; Vol. 34, No. 1-2 SPEC. ISS. pp. 51-62.
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