Lipase incorporated ionic liquid polymers as active, stable and reusable biocatalysts

Muhammad Moniruzzaman, Keishirou Ino, Noriho Kamiya, Masahiro Goto

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The aim of this study was to develop ionic liquid (IL) polymer materials incorporating enzymes that can be used as active, stable and reusable biocatalysts. To this goal, Candida rugosa lipase has been microencapsulated in surfactant aggregates formed in an IL monomer or the solution of an IL monomer/IL and then incorporated into polymer frameworks through the free radical polymerization of an IL (1-vinyl-3-ethylimidazolium bis(trifluoromethyl- sulfonyl) amide) ([veim][Tf2N]). The activity, stability and reusability of such IL polymer materials containing lipase were evaluated using lipase-catalyzed hydrolysis of p-nitrophenyl butyrate (p-PNB) as a model reaction. Lipase encapsulated within ionic liquid polymer materials remained active and exhibited excellent stability in aqueous solutions. More importantly, these biopolymer materials retained most of their activity after five reaction cycles, in which biopolymers were recovered from the reaction mixture simply by centrifugation. This study promulgates a direction toward the design of IL-an interesting class of tunable and designable solvents-based polymer materials containing biomolecules via a combination of polymer and supramolecular chemistry for diverse applications.

Original languageEnglish
Pages (from-to)7707-7713
Number of pages7
JournalOrganic and Biomolecular Chemistry
Volume10
Issue number38
DOIs
Publication statusPublished - Oct 14 2012

Fingerprint

Ionic Liquids
Lipase
Polymers
polymers
Enzymes
liquids
Biopolymers
biopolymers
monomers
Monomers
Supramolecular chemistry
polymer chemistry
Centrifugation
Candida
Reusability
Biomolecules
Free radical polymerization
Surface-Active Agents
Amides
Polymerization

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Lipase incorporated ionic liquid polymers as active, stable and reusable biocatalysts. / Moniruzzaman, Muhammad; Ino, Keishirou; Kamiya, Noriho; Goto, Masahiro.

In: Organic and Biomolecular Chemistry, Vol. 10, No. 38, 14.10.2012, p. 7707-7713.

Research output: Contribution to journalArticle

@article{386e7c29b0a94f94b6b77c4569471f40,
title = "Lipase incorporated ionic liquid polymers as active, stable and reusable biocatalysts",
abstract = "The aim of this study was to develop ionic liquid (IL) polymer materials incorporating enzymes that can be used as active, stable and reusable biocatalysts. To this goal, Candida rugosa lipase has been microencapsulated in surfactant aggregates formed in an IL monomer or the solution of an IL monomer/IL and then incorporated into polymer frameworks through the free radical polymerization of an IL (1-vinyl-3-ethylimidazolium bis(trifluoromethyl- sulfonyl) amide) ([veim][Tf2N]). The activity, stability and reusability of such IL polymer materials containing lipase were evaluated using lipase-catalyzed hydrolysis of p-nitrophenyl butyrate (p-PNB) as a model reaction. Lipase encapsulated within ionic liquid polymer materials remained active and exhibited excellent stability in aqueous solutions. More importantly, these biopolymer materials retained most of their activity after five reaction cycles, in which biopolymers were recovered from the reaction mixture simply by centrifugation. This study promulgates a direction toward the design of IL-an interesting class of tunable and designable solvents-based polymer materials containing biomolecules via a combination of polymer and supramolecular chemistry for diverse applications.",
author = "Muhammad Moniruzzaman and Keishirou Ino and Noriho Kamiya and Masahiro Goto",
year = "2012",
month = "10",
day = "14",
doi = "10.1039/c2ob25529d",
language = "English",
volume = "10",
pages = "7707--7713",
journal = "Organic and Biomolecular Chemistry",
issn = "1477-0520",
publisher = "Royal Society of Chemistry",
number = "38",

}

TY - JOUR

T1 - Lipase incorporated ionic liquid polymers as active, stable and reusable biocatalysts

AU - Moniruzzaman, Muhammad

AU - Ino, Keishirou

AU - Kamiya, Noriho

AU - Goto, Masahiro

PY - 2012/10/14

Y1 - 2012/10/14

N2 - The aim of this study was to develop ionic liquid (IL) polymer materials incorporating enzymes that can be used as active, stable and reusable biocatalysts. To this goal, Candida rugosa lipase has been microencapsulated in surfactant aggregates formed in an IL monomer or the solution of an IL monomer/IL and then incorporated into polymer frameworks through the free radical polymerization of an IL (1-vinyl-3-ethylimidazolium bis(trifluoromethyl- sulfonyl) amide) ([veim][Tf2N]). The activity, stability and reusability of such IL polymer materials containing lipase were evaluated using lipase-catalyzed hydrolysis of p-nitrophenyl butyrate (p-PNB) as a model reaction. Lipase encapsulated within ionic liquid polymer materials remained active and exhibited excellent stability in aqueous solutions. More importantly, these biopolymer materials retained most of their activity after five reaction cycles, in which biopolymers were recovered from the reaction mixture simply by centrifugation. This study promulgates a direction toward the design of IL-an interesting class of tunable and designable solvents-based polymer materials containing biomolecules via a combination of polymer and supramolecular chemistry for diverse applications.

AB - The aim of this study was to develop ionic liquid (IL) polymer materials incorporating enzymes that can be used as active, stable and reusable biocatalysts. To this goal, Candida rugosa lipase has been microencapsulated in surfactant aggregates formed in an IL monomer or the solution of an IL monomer/IL and then incorporated into polymer frameworks through the free radical polymerization of an IL (1-vinyl-3-ethylimidazolium bis(trifluoromethyl- sulfonyl) amide) ([veim][Tf2N]). The activity, stability and reusability of such IL polymer materials containing lipase were evaluated using lipase-catalyzed hydrolysis of p-nitrophenyl butyrate (p-PNB) as a model reaction. Lipase encapsulated within ionic liquid polymer materials remained active and exhibited excellent stability in aqueous solutions. More importantly, these biopolymer materials retained most of their activity after five reaction cycles, in which biopolymers were recovered from the reaction mixture simply by centrifugation. This study promulgates a direction toward the design of IL-an interesting class of tunable and designable solvents-based polymer materials containing biomolecules via a combination of polymer and supramolecular chemistry for diverse applications.

UR - http://www.scopus.com/inward/record.url?scp=84874848950&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874848950&partnerID=8YFLogxK

U2 - 10.1039/c2ob25529d

DO - 10.1039/c2ob25529d

M3 - Article

C2 - 22903458

AN - SCOPUS:84874848950

VL - 10

SP - 7707

EP - 7713

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

SN - 1477-0520

IS - 38

ER -