Lipid raft-associated catechin suppresses the FcεRI expression by inhibiting phosphorylation of the extracellular signal-regulated kinase1/2

Yoshinori Fujimura, Hirofumi Tachibana, Koji Yamada

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The major green tea catechin, (-)-epigallocatechin-3-O-gallate (EGCG), has a suppressive effect on the expression of the high-affinity IgE receptor FcεRI, which is key molecule in the IgE-mediated allergic reactions. Here we show that EGCG binds to the cell surface and highly associates with plasma membrane microdomains, lipid rafts, on the human basophilic KU812 cells. The disruption of these lipid rafts caused a reduction of the amount of raft-associated EGCG and the FcεRI-suppressive effect of EGCG. We also found that EGCG has an ability to inhibit the phosphorylation of the extracellular signal-regulated kinase1/2 (ERK1/2) and that the ERK1/2 specific inhibitor also reduced FcεRI expression. Moreover, the inhibitory effect elicited by EGCG on ERK1/2 was prevented by disruption of rafts. Thus, these results suggest that the interaction between EGCG and the lipid rafts is important for EGCG's ability to downregulate FcεRI expression, and ERK1/2 may be involved in this suppression signal.

Original languageEnglish
Pages (from-to)204-210
Number of pages7
JournalFEBS Letters
Volume556
Issue number1-3
DOIs
Publication statusPublished - Jan 2 2004

Fingerprint

Phosphorylation
Catechin
Lipids
Membrane Microdomains
IgE Receptors
Tea
Cell membranes
Membrane Lipids
epigallocatechin gallate
Immunoglobulin E
Hypersensitivity
Down-Regulation
Cell Membrane
Molecules

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Lipid raft-associated catechin suppresses the FcεRI expression by inhibiting phosphorylation of the extracellular signal-regulated kinase1/2. / Fujimura, Yoshinori; Tachibana, Hirofumi; Yamada, Koji.

In: FEBS Letters, Vol. 556, No. 1-3, 02.01.2004, p. 204-210.

Research output: Contribution to journalArticle

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N2 - The major green tea catechin, (-)-epigallocatechin-3-O-gallate (EGCG), has a suppressive effect on the expression of the high-affinity IgE receptor FcεRI, which is key molecule in the IgE-mediated allergic reactions. Here we show that EGCG binds to the cell surface and highly associates with plasma membrane microdomains, lipid rafts, on the human basophilic KU812 cells. The disruption of these lipid rafts caused a reduction of the amount of raft-associated EGCG and the FcεRI-suppressive effect of EGCG. We also found that EGCG has an ability to inhibit the phosphorylation of the extracellular signal-regulated kinase1/2 (ERK1/2) and that the ERK1/2 specific inhibitor also reduced FcεRI expression. Moreover, the inhibitory effect elicited by EGCG on ERK1/2 was prevented by disruption of rafts. Thus, these results suggest that the interaction between EGCG and the lipid rafts is important for EGCG's ability to downregulate FcεRI expression, and ERK1/2 may be involved in this suppression signal.

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