Lipidomic analysis of plasma lipoprotein fractions in myocardial infarction-prone rabbits

Hiroaki Takeda, Tomonari Koike, Yoshihiro Izumi, Takayuki Yamada, Masaru Yoshida, Masashi Shiomi, Eiichiro Fukusaki, Takeshi Bamba

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Lipids play important roles in the body and are transported to various tissues via lipoproteins. It is commonly assumed that alteration of lipid levels in lipoproteins leads to dyslipidemia and serious diseases such as coronary artery disease (CAD). However, lipid compositions in each lipoprotein fraction induced by lipoprotein metabolism are poorly understood. Lipidomics, which involves the comprehensive and quantitative analysis of lipids, is expected to provide valuable information regarding the pathogenic mechanism of CAD. Here, we performed a lipidomic analysis of plasma and its lipoprotein fractions in myocardial infarction-prone Watanabe heritable hyperlipidemic (WHHLMI) rabbits. In total, 172 lipids in plasma obtained from normal and WHHLMI rabbits were quantified with high throughput and accuracy using supercritical fluid chromatography hybrid quadrupole-Orbitrap mass spectrometry (SFC/Q-Orbitrap-MS). Plasma levels of each lipid class (i.e., phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, lysophosphatidylcholine, lysophosphatidylethanolamine, sphingomyelin, ceramide, triacylglycerol, diacylglycerol, and cholesterol ester, except for free fatty acids) in 21-month-old WHHLMI rabbits were significantly higher than those in normal rabbits. High levels of functional lipids, such as alkyl-phosphatidylcholines, phospholipids including ω-6 fatty acids, and plasmalogens, were also observed in WHHLMI rabbit plasma. In addition, high-resolution lipidomic analysis using very low density lipoprotein (VLDL) and low density lipoprotein (LDL) provided information on the specific molecular species of lipids in each lipoprotein fraction. In particular, higher levels of phosphatidylethanolamine plasmalogens were detected in LDL than in VLDL. Our lipidomics approach for plasma lipoprotein fractions will be useful for in-depth studies on the pathogenesis of CAD.

Original languageEnglish
Pages (from-to)476-482
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume120
Issue number4
DOIs
Publication statusPublished - Oct 1 2015

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Lipoproteins
Myocardial Infarction
Rabbits
Lipids
Plasmas
Coronary Artery Disease
VLDL Lipoproteins
Phosphatidylcholines
LDL Lipoproteins
Fatty acids
Plasmalogens
Supercritical Fluid Chromatography
Lysophosphatidylcholines
Sphingomyelins
Cholesterol Esters
Ceramides
Diglycerides
Dyslipidemias
Phosphatidylinositols
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Lipidomic analysis of plasma lipoprotein fractions in myocardial infarction-prone rabbits. / Takeda, Hiroaki; Koike, Tomonari; Izumi, Yoshihiro; Yamada, Takayuki; Yoshida, Masaru; Shiomi, Masashi; Fukusaki, Eiichiro; Bamba, Takeshi.

In: Journal of Bioscience and Bioengineering, Vol. 120, No. 4, 01.10.2015, p. 476-482.

Research output: Contribution to journalArticle

Takeda, Hiroaki ; Koike, Tomonari ; Izumi, Yoshihiro ; Yamada, Takayuki ; Yoshida, Masaru ; Shiomi, Masashi ; Fukusaki, Eiichiro ; Bamba, Takeshi. / Lipidomic analysis of plasma lipoprotein fractions in myocardial infarction-prone rabbits. In: Journal of Bioscience and Bioengineering. 2015 ; Vol. 120, No. 4. pp. 476-482.
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