Lipopolysaccharide and granulocyte colony-stimulating factor delay neutrophil apoptosis and ingestion by guinea pig macrophages

C. Yamamoto, S. I. Yoshida, Hatsumi Taniguchi, Hui Qin Ming Hui Qin, H. Miyamoto, Y. Mizuguchi

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Abstract

In previous studies, neutrophil-ingesting macrophages were clearly and easily observed in the peritoneal cavity of guinea pigs after intraperitoneal injection of thioglycolate medium, and phagocytosis of neutrophils by macrophages could be detected in in vitro cultures of peritoneal exudate cells. Using an in vitro system, we examined the effect of bacterial lipopolysaccharide and recombinant human granulocyte colony-stimulating factor on the apoptosis (programmed cell death) of neutrophils and their subsequent ingestion by macrophages. Lipopolysaccharide delayed karyopyknosis and apoptosis of neutrophils, as shown by endogenous endonuclease activity and a high proportion of trypan blue-excluding cells, and subsequent ingestion by autologous macrophages. Granulocyte colony-stimulating factor also delayed neutrophil karyopyknosis and ingestion by macrophages. When a thioglycolate medium was coinjected intraperitoneally with lipopolysaccharide into guinea pigs in the in vivo system, delays in neutrophil disappearance and ingestion by macrophages in the peritoneal cavity were also observed. We suggest that bacterial products and cytokines regulate neutrophil apoptosis and subsequent ingestion by macrophages at inflamed sites.

Original languageEnglish
Pages (from-to)1972-1979
Number of pages8
JournalInfection and Immunity
Volume61
Issue number5
Publication statusPublished - 1993
Externally publishedYes

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Granulocyte Colony-Stimulating Factor
Lipopolysaccharides
Guinea Pigs
Neutrophils
Eating
Macrophages
Apoptosis
Thioglycolates
Peritoneal Cavity
Trypan Blue
Endonucleases
Exudates and Transudates
Intraperitoneal Injections
Phagocytosis
Cell Death
Cytokines

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Yamamoto, C., Yoshida, S. I., Taniguchi, H., Ming Hui Qin, H. Q., Miyamoto, H., & Mizuguchi, Y. (1993). Lipopolysaccharide and granulocyte colony-stimulating factor delay neutrophil apoptosis and ingestion by guinea pig macrophages. Infection and Immunity, 61(5), 1972-1979.

Lipopolysaccharide and granulocyte colony-stimulating factor delay neutrophil apoptosis and ingestion by guinea pig macrophages. / Yamamoto, C.; Yoshida, S. I.; Taniguchi, Hatsumi; Ming Hui Qin, Hui Qin; Miyamoto, H.; Mizuguchi, Y.

In: Infection and Immunity, Vol. 61, No. 5, 1993, p. 1972-1979.

Research output: Contribution to journalArticle

Yamamoto, C, Yoshida, SI, Taniguchi, H, Ming Hui Qin, HQ, Miyamoto, H & Mizuguchi, Y 1993, 'Lipopolysaccharide and granulocyte colony-stimulating factor delay neutrophil apoptosis and ingestion by guinea pig macrophages', Infection and Immunity, vol. 61, no. 5, pp. 1972-1979.
Yamamoto, C. ; Yoshida, S. I. ; Taniguchi, Hatsumi ; Ming Hui Qin, Hui Qin ; Miyamoto, H. ; Mizuguchi, Y. / Lipopolysaccharide and granulocyte colony-stimulating factor delay neutrophil apoptosis and ingestion by guinea pig macrophages. In: Infection and Immunity. 1993 ; Vol. 61, No. 5. pp. 1972-1979.
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