Lipopolysaccharide binding of the mite allergen Der f 2

Saori Ichikawa, Toshiro Takai, Tomoe Yashiki, Seizo Takahashi, Ko Okumura, Hideoki Ogawa, Daisuke Kohda, Hideki Hatanaka

    Research output: Contribution to journalArticle

    57 Citations (Scopus)

    Abstract

    Lipid-binding properties and/or involvement with host defense are often found in allergen proteins, implying that these intrinsic biological functions likely contribute to the allergenicity of allergens. The group 2 major mite allergens, Der f 2 and Der p 2, show structural homology with MD-2, the lipopolysaccharide (LPS)-binding component of the Toll-like receptor (TLR) 4 signalling complex. Elucidation of the ligand-binding properties of group 2 mite allergens and identification of interaction sites by structural studies are important to explore the relationship between allergenicity and biological function. Here, we report a ligand-fishing approach in which His-tagged Der f 2 was incubated with sonicated stable isotope-labelled Escherichia coli as a potential ligand source, followed by isolation of Der f 2-bound material by a HisTrap column and NMR analysis. We found that Der f 2 binds to LPS with a nanomolar affinity and, using fluorescence and gel filtration assays that LPS binds to Der f 2 in a molar ratio of 1:1. We mapped the LPS-binding interface of Der f 2 by NMR perturbation studies, which suggested that LPS binds Der f 2 between the two large β-sheets, similar to its binding to MD-2, the LPS-binding component of the innate immunity receptor TLR4.

    Original languageEnglish
    Pages (from-to)1055-1065
    Number of pages11
    JournalGenes to Cells
    Volume14
    Issue number9
    DOIs
    Publication statusPublished - Sep 10 2009

    Fingerprint

    Mites
    Lipopolysaccharides
    Allergens
    Toll-Like Receptor 4
    Ligands
    Dermatophagoides farinae antigen f 2
    Innate Immunity
    Isotopes
    Gel Chromatography
    Fluorescence
    Escherichia coli
    Lipids
    Proteins

    All Science Journal Classification (ASJC) codes

    • Genetics
    • Cell Biology

    Cite this

    Ichikawa, S., Takai, T., Yashiki, T., Takahashi, S., Okumura, K., Ogawa, H., ... Hatanaka, H. (2009). Lipopolysaccharide binding of the mite allergen Der f 2. Genes to Cells, 14(9), 1055-1065. https://doi.org/10.1111/j.1365-2443.2009.01334.x

    Lipopolysaccharide binding of the mite allergen Der f 2. / Ichikawa, Saori; Takai, Toshiro; Yashiki, Tomoe; Takahashi, Seizo; Okumura, Ko; Ogawa, Hideoki; Kohda, Daisuke; Hatanaka, Hideki.

    In: Genes to Cells, Vol. 14, No. 9, 10.09.2009, p. 1055-1065.

    Research output: Contribution to journalArticle

    Ichikawa, S, Takai, T, Yashiki, T, Takahashi, S, Okumura, K, Ogawa, H, Kohda, D & Hatanaka, H 2009, 'Lipopolysaccharide binding of the mite allergen Der f 2', Genes to Cells, vol. 14, no. 9, pp. 1055-1065. https://doi.org/10.1111/j.1365-2443.2009.01334.x
    Ichikawa S, Takai T, Yashiki T, Takahashi S, Okumura K, Ogawa H et al. Lipopolysaccharide binding of the mite allergen Der f 2. Genes to Cells. 2009 Sep 10;14(9):1055-1065. https://doi.org/10.1111/j.1365-2443.2009.01334.x
    Ichikawa, Saori ; Takai, Toshiro ; Yashiki, Tomoe ; Takahashi, Seizo ; Okumura, Ko ; Ogawa, Hideoki ; Kohda, Daisuke ; Hatanaka, Hideki. / Lipopolysaccharide binding of the mite allergen Der f 2. In: Genes to Cells. 2009 ; Vol. 14, No. 9. pp. 1055-1065.
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