Lipopolysaccharide (LPS) increases the invasive ability of pancreatic cancer cells through the TLR4/MyD88 signaling pathway

Mio Ikebe, Yoshiki Kitaura, Masafumi Nakamura, Haruo Tanaka, Akio Yamasaki, Shuntaro Nagai, Junji Wada, Kosuke Yanai, Kenichiro Koga, Norihiro Sato, Makoto Kubo, Masao Tanaka, Hideya Ohnishi, Mitsuo Katano

Research output: Contribution to journalArticle

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Abstract

Background: Inflammation plays a multifaceted role in cancer progression, and NF-κB is one of the key factors connecting inflammation with cancer progression. We have shown that lipopolysaccharide (LPS) promotes NF-κB activation in colon cancer cells and pancreatic cancer cells. However, it is unclear why inflammatory stimuli can induce NF-κB activation in cancer cells. Methods:We used two human pancreatic cancer cells, Panc-1 and AsPC-1, as target cells. LPS was used as an inflammatory stimulus. To confirm the participation of TLR4/NF-κB signaling pathway, we used three different NF-κB inhibitors (PDTC, IkBa mutant, and NF-κB decoy ODN) and siRNAs (against TLR4, MyD88, and MMP-9). Effect of LPS on pancreatic cancer cell invasive ability was determined by Matrigel invasion assay. Results: LPS increased the invasive ability of pancreatic cancer cells, while blockade of NF-κB pathway decreased the LPS-dependent increased invasive ability. Blockade of TLR4 or MyD88 by siRNA also decreased the LPS-dependent increased invasive ability. Conclusion: These results suggest that TLR/MyD88/NF-κB signaling pathway plays a significant role in connecting inflammation and cancer invasion and progression.

Original languageEnglish
Pages (from-to)725-731
Number of pages7
JournalJournal of Surgical Oncology
Volume100
Issue number8
DOIs
Publication statusPublished - Dec 15 2009

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Pancreatic Neoplasms
Lipopolysaccharides
Inflammation
Neoplasms
Matrix Metalloproteinases
Colonic Neoplasms
Small Interfering RNA

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oncology

Cite this

Lipopolysaccharide (LPS) increases the invasive ability of pancreatic cancer cells through the TLR4/MyD88 signaling pathway. / Ikebe, Mio; Kitaura, Yoshiki; Nakamura, Masafumi; Tanaka, Haruo; Yamasaki, Akio; Nagai, Shuntaro; Wada, Junji; Yanai, Kosuke; Koga, Kenichiro; Sato, Norihiro; Kubo, Makoto; Tanaka, Masao; Ohnishi, Hideya; Katano, Mitsuo.

In: Journal of Surgical Oncology, Vol. 100, No. 8, 15.12.2009, p. 725-731.

Research output: Contribution to journalArticle

Ikebe, Mio ; Kitaura, Yoshiki ; Nakamura, Masafumi ; Tanaka, Haruo ; Yamasaki, Akio ; Nagai, Shuntaro ; Wada, Junji ; Yanai, Kosuke ; Koga, Kenichiro ; Sato, Norihiro ; Kubo, Makoto ; Tanaka, Masao ; Ohnishi, Hideya ; Katano, Mitsuo. / Lipopolysaccharide (LPS) increases the invasive ability of pancreatic cancer cells through the TLR4/MyD88 signaling pathway. In: Journal of Surgical Oncology. 2009 ; Vol. 100, No. 8. pp. 725-731.
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AU - Tanaka, Haruo

AU - Yamasaki, Akio

AU - Nagai, Shuntaro

AU - Wada, Junji

AU - Yanai, Kosuke

AU - Koga, Kenichiro

AU - Sato, Norihiro

AU - Kubo, Makoto

AU - Tanaka, Masao

AU - Ohnishi, Hideya

AU - Katano, Mitsuo

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