Lipopolysaccharides from Campylobacter jejuni associated with Guillain- Barre syndrome patients mimic human gangliosides in structure

G. O. Aspinall, Shuji Fujimoto, A. G. McDonald, H. Pang, L. A. Kurjanczyk, J. L. Penner

Research output: Contribution to journalComment/debate

142 Citations (Scopus)

Abstract

Lipopolysaccharides extracted from Campylobacter jejuni serostrains (serotype reference strains) for serotypes O:4 and O:19 were found to have core oligosaccharides with terminal structures resembling human gangliosides G(M1) and G(D1a). High-molecular-weight molecules that reflected the presence of O chains were shown in immunoblots to be immunologically specific for each serostrain. The O:19 antiserum also reacted strongly with core oligosaccharides of two isolates from patients with Guillain-Barre syndrome (GBS), but the banding patterns and molecular structures were different from those of the O:19 serostrain. A neuraminobiose disaccharide unit is attached to the terminal Gal residue in one isolate, and the other isolate lacked terminal N-acetyl glucosamine and galactose with attached sialic acid so that the sialic acid residues were present in a neuraminobiose unit linked to the only remaining galactose. Analysis of the high-M(r) lipopolysaccharides of the O:19 serostrain and the two isolates from GBS patients revealed the presence of a hyaluronic acid-like polymer with disaccharide-repeating units consisting of β-D-glucuronic acid amidated with 2-amino-2-deoxyglycerol and N-acetyl glucosamine. The results confirm a potential role for the core oligosaccharides in the etiology of GBS but also suggest that the O-chain polysaccharide may be a contributing factor.

Original languageEnglish
Pages (from-to)2122-2125
Number of pages4
JournalInfection and Immunity
Volume62
Issue number5
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Campylobacter jejuni
Guillain-Barre Syndrome
Gangliosides
Lipopolysaccharides
Oligosaccharides
Disaccharides
Glucosamine
N-Acetylneuraminic Acid
Galactose
G(M1) Ganglioside
Glucuronic Acid
Hyaluronic Acid
Molecular Structure
Polysaccharides
Immune Sera
Polymers
Molecular Weight
Serogroup

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Aspinall, G. O., Fujimoto, S., McDonald, A. G., Pang, H., Kurjanczyk, L. A., & Penner, J. L. (1994). Lipopolysaccharides from Campylobacter jejuni associated with Guillain- Barre syndrome patients mimic human gangliosides in structure. Infection and Immunity, 62(5), 2122-2125.

Lipopolysaccharides from Campylobacter jejuni associated with Guillain- Barre syndrome patients mimic human gangliosides in structure. / Aspinall, G. O.; Fujimoto, Shuji; McDonald, A. G.; Pang, H.; Kurjanczyk, L. A.; Penner, J. L.

In: Infection and Immunity, Vol. 62, No. 5, 1994, p. 2122-2125.

Research output: Contribution to journalComment/debate

Aspinall, GO, Fujimoto, S, McDonald, AG, Pang, H, Kurjanczyk, LA & Penner, JL 1994, 'Lipopolysaccharides from Campylobacter jejuni associated with Guillain- Barre syndrome patients mimic human gangliosides in structure', Infection and Immunity, vol. 62, no. 5, pp. 2122-2125.
Aspinall, G. O. ; Fujimoto, Shuji ; McDonald, A. G. ; Pang, H. ; Kurjanczyk, L. A. ; Penner, J. L. / Lipopolysaccharides from Campylobacter jejuni associated with Guillain- Barre syndrome patients mimic human gangliosides in structure. In: Infection and Immunity. 1994 ; Vol. 62, No. 5. pp. 2122-2125.
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