Liposomal membranes: VI. polysaccharide-induced aggregation of multilamellar liposomes of egg lecithin

Junzo Sunamoto, Kiyoshi Iwamoto, Hiroki Kondo, Seiji Shinkai

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Induced aggregation of multilamellar liposomes of egg lecithin with several polysaccharides, such as pullulan, dextran, hydroxyethylstarch, and amylopectin, has been investigated in conjunction with structural characteristics of polysaccharides, incubation temperature, ionic strength, and surface charge of liposomes. Aggregation of liposomes as a function of time was followed by monitoring turbidity of the liposome suspension at 600 nm. The inverse relationship between the minimal concentration and molecular weight of polysaccharides for the induced aggregation of liposomes was observed for all the polysaccharides employed. Polysaccharides having larger excluded volume more easily bind to liposomes, leading to the acceleration of the aggregation of liposomes. Hence, the order of efficiency of polysac charides for the promotion of liposome aggregation was pullulan>dextran>hydroxyethyl starch≃amylopectin Increasing the surface charge of liposomes brought about a decrease in aggregation irrespective of the sign. Even when negatively charged liposomes were incu-, bated with the cationic DEAE-dextran, the liposome aggregation was depressed. These results including salt effect and temperature dependency of the induced aggregation of lipo somes supported a plausible mechanism for the reaction, the polysaccharide-bridging mech anism, which has been provided for the polysaccharide-promoted agglutination of human erythrocytes.

Original languageEnglish
Pages (from-to)1219-1226
Number of pages8
JournalJournal of biochemistry
Volume88
Issue number5
DOIs
Publication statusPublished - Jan 1 1980

Fingerprint

Lecithins
Liposomes
Polysaccharides
Ovum
Agglomeration
Membranes
Surface charge
Dextrans
Amylopectin
DEAE-Dextran
Temperature
Agglutination
Turbidity
Ionic strength
Osmolar Concentration
Suspensions
Salts
Erythrocytes
Molecular Weight
Molecular weight

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Liposomal membranes : VI. polysaccharide-induced aggregation of multilamellar liposomes of egg lecithin. / Sunamoto, Junzo; Iwamoto, Kiyoshi; Kondo, Hiroki; Shinkai, Seiji.

In: Journal of biochemistry, Vol. 88, No. 5, 01.01.1980, p. 1219-1226.

Research output: Contribution to journalArticle

Sunamoto, Junzo ; Iwamoto, Kiyoshi ; Kondo, Hiroki ; Shinkai, Seiji. / Liposomal membranes : VI. polysaccharide-induced aggregation of multilamellar liposomes of egg lecithin. In: Journal of biochemistry. 1980 ; Vol. 88, No. 5. pp. 1219-1226.
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