Lipoteichoic acids from Lactobacillus strains elicit strong tumor necrosis factor alpha-inducing activities in macrophages through toll-like receptor 2

Tetsuya Matsuguchi, Akimitsu Takagi, Takeshi Matsuzaki, Masato Nagaoka, Kimika Ishikawa, Teruo Yokokura, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    164 Citations (Scopus)

    Abstract

    Lactobacilli are nonpathogenic gram-positive inhabitants of microflora. At least some Lactobacillus strains have been postulated to have health beneficial effects, such as the stimulation of the immune system. Here we examined the stimulatory effects of lactobacilli on mouse immune cells. All six heat-killed Lactobacillus strains examined induced the secretion of tumor necrosis factor alpha (TNF-α) from mouse splenic mononuclear cells, albeit to various degrees. When fractionated subcellular fractions of Lactobacillus casei were tested for NF-κB activation and TNF-α production in RAW264.7, a mouse macrophage cell line, the activity was found to be as follows: protoplast > cell wall ≫ polysaccharide-peptidoglycan complex. Both crude extracts and purified lipoteichoic acids (LTAs) from two Lactobacillus strains, L. casei and L. fermentum, significantly induced TNF-α secretion from RAW264.7 cells and splenocytes of C57BL/6, C3H/HeN, and C3H/HeJ mice but not from splenocytes of C57BL/6 TLR2-/- mice. Lactobacillus LTA induced activation of c-Jun N-terminal kinase activation in RAW264.7 cells. Furthermore, in HEK293T cells transected with a combination of CD14 and Toll-like receptor 2 (TLR2), NF-κB was activated in response to Lactobacillus LTA. Taken together, these data suggest that LTAs from lactobacilli elicit proinflammatory activities through TLR2.

    Original languageEnglish
    Pages (from-to)259-266
    Number of pages8
    JournalClinical and Diagnostic Laboratory Immunology
    Volume10
    Issue number2
    DOIs
    Publication statusPublished - Mar 1 2003

    Fingerprint

    Toll-Like Receptor 2
    Macrophages
    Lactobacillus
    Tumor Necrosis Factor-alpha
    Chemical activation
    Cells
    Lactobacillus casei
    Peptidoglycan
    JNK Mitogen-Activated Protein Kinases
    Immune system
    Complex Mixtures
    Polysaccharides
    Health
    Subcellular Fractions
    lipoteichoic acid
    Inbred C3H Mouse
    Protoplasts
    Cell Wall
    Immune System
    Hot Temperature

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology
    • Clinical Biochemistry
    • Microbiology (medical)

    Cite this

    Lipoteichoic acids from Lactobacillus strains elicit strong tumor necrosis factor alpha-inducing activities in macrophages through toll-like receptor 2. / Matsuguchi, Tetsuya; Takagi, Akimitsu; Matsuzaki, Takeshi; Nagaoka, Masato; Ishikawa, Kimika; Yokokura, Teruo; Yoshikai, Yasunobu.

    In: Clinical and Diagnostic Laboratory Immunology, Vol. 10, No. 2, 01.03.2003, p. 259-266.

    Research output: Contribution to journalArticle

    Matsuguchi, Tetsuya ; Takagi, Akimitsu ; Matsuzaki, Takeshi ; Nagaoka, Masato ; Ishikawa, Kimika ; Yokokura, Teruo ; Yoshikai, Yasunobu. / Lipoteichoic acids from Lactobacillus strains elicit strong tumor necrosis factor alpha-inducing activities in macrophages through toll-like receptor 2. In: Clinical and Diagnostic Laboratory Immunology. 2003 ; Vol. 10, No. 2. pp. 259-266.
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