TY - JOUR
T1 - Liquid chromatography-fast atom bombardment mass spectrometry for detection and determination of pentazocine in human tissues
AU - Imamura, Tohru
AU - Kudo, Keiko
AU - Namera, Akira
AU - Tokunaga, Hironori
AU - Yashiki, Mikio
AU - Jitsufuchi, Narumi
AU - Kojima, Tohru
N1 - Funding Information:
This research was partly supported by a Grant-in-Aid for the Encouragement for Young Scientists (No. 10770191) from the Ministry of Education, Science and Culture, Japan.
PY - 1999/8/6
Y1 - 1999/8/6
N2 - A reliable and sensitive method was developed for the detection and determination of pentazocine in human solid tissues using liquid chromatography-dynamic fast atom bombardment (FAB) mass spectrometry, combined with a three-step liquid-liquid extraction procedure. Levallorphan tartrate served as an internal standard. The extract was evaporated to dryness and dissolved in the mobile phase, acetonitrile-10 mM ammonium acetate solution (20:80, pH 4.0) containing 0.5% glycerol as FAB matrix. The eluent was pumped at a flow rate of 25 μl/min and split before introduction to FAB mass spectrometer. Quantitative analysis was carried out by means of monitoring quasi-molecular ions with m/z 286 for pentazocine and m/z 284 for levallorphan. The lower limit of detection of pentazocine in each tissue tested was 1 ng/g with scan mode and 0.1 ng/g with SIM mode. Using this method, the concentrations of pentazocine were determined in the tissues of an autopsied individual to perform toxicological evaluation. Copyright (C) 1999 Elsevier Science B.V.
AB - A reliable and sensitive method was developed for the detection and determination of pentazocine in human solid tissues using liquid chromatography-dynamic fast atom bombardment (FAB) mass spectrometry, combined with a three-step liquid-liquid extraction procedure. Levallorphan tartrate served as an internal standard. The extract was evaporated to dryness and dissolved in the mobile phase, acetonitrile-10 mM ammonium acetate solution (20:80, pH 4.0) containing 0.5% glycerol as FAB matrix. The eluent was pumped at a flow rate of 25 μl/min and split before introduction to FAB mass spectrometer. Quantitative analysis was carried out by means of monitoring quasi-molecular ions with m/z 286 for pentazocine and m/z 284 for levallorphan. The lower limit of detection of pentazocine in each tissue tested was 1 ng/g with scan mode and 0.1 ng/g with SIM mode. Using this method, the concentrations of pentazocine were determined in the tissues of an autopsied individual to perform toxicological evaluation. Copyright (C) 1999 Elsevier Science B.V.
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U2 - 10.1016/S0378-4347(99)00152-8
DO - 10.1016/S0378-4347(99)00152-8
M3 - Article
C2 - 10492001
AN - SCOPUS:0032801767
SN - 0378-4347
VL - 731
SP - 149
EP - 154
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 1
ER -