Liquid-liquid extraction of enzymatically synthesized functional RNA oligonucleotides using reverse micelles with a DNA-surfactant

Tatsuo Maruyama, Naoki Ishizu, Yuka Eguchi, Takuya Hosogi, Masahiro Goto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We successfully implemented solvent extraction of short, single-stranded RNA using reverse micelles (water-in-oil microemulsions) with a DNA-surfactant. A thrombin-binding RNA aptamer was enzymatically synthesized and purified by extraction using the reverse micellar system. The extracted RNA aptamer retained thrombin-binding activity after the extraction procedure.

Original languageEnglish
Pages (from-to)12376-12379
Number of pages4
JournalChemical Communications
Volume52
Issue number83
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Nucleotide Aptamers
Oligonucleotides
Micelles
RNA
Surface-Active Agents
Thrombin
Surface active agents
DNA
Microemulsions
Liquids
Solvent extraction
Oils
Water

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Liquid-liquid extraction of enzymatically synthesized functional RNA oligonucleotides using reverse micelles with a DNA-surfactant. / Maruyama, Tatsuo; Ishizu, Naoki; Eguchi, Yuka; Hosogi, Takuya; Goto, Masahiro.

In: Chemical Communications, Vol. 52, No. 83, 01.01.2016, p. 12376-12379.

Research output: Contribution to journalArticle

Maruyama, Tatsuo ; Ishizu, Naoki ; Eguchi, Yuka ; Hosogi, Takuya ; Goto, Masahiro. / Liquid-liquid extraction of enzymatically synthesized functional RNA oligonucleotides using reverse micelles with a DNA-surfactant. In: Chemical Communications. 2016 ; Vol. 52, No. 83. pp. 12376-12379.
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