Liquid-liquid extraction of enzymatically synthesized functional RNA oligonucleotides using reverse micelles with a DNA-surfactant

Tatsuo Maruyama, Naoki Ishizu, Yuka Eguchi, Takuya Hosogi, Masahiro Goto

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    We successfully implemented solvent extraction of short, single-stranded RNA using reverse micelles (water-in-oil microemulsions) with a DNA-surfactant. A thrombin-binding RNA aptamer was enzymatically synthesized and purified by extraction using the reverse micellar system. The extracted RNA aptamer retained thrombin-binding activity after the extraction procedure.

    Original languageEnglish
    Pages (from-to)12376-12379
    Number of pages4
    JournalChemical Communications
    Volume52
    Issue number83
    DOIs
    Publication statusPublished - 2016

    All Science Journal Classification (ASJC) codes

    • Catalysis
    • Electronic, Optical and Magnetic Materials
    • Ceramics and Composites
    • Chemistry(all)
    • Surfaces, Coatings and Films
    • Metals and Alloys
    • Materials Chemistry

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