Liquid membrane transport of nucleotides by a quaternary ammonium salt

Fukiko Kubota, Hisashi Yamada, Masahiro Goto, Shintaro Furusaki

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Transport of adenosine nucleotides across a bulk liquid membrane was examined by using a long-chain quaternary ammonium salt as a mobile carrier. In order to construct the separation system avoiding the use of organic chloride solvents, distearyldimethylammonium chloride, which is rendered soluble in an aliphatic solvent such as isooctane by a small addition of alcohol, was employed as the carrier, and the successful transport of nucleotides was achieved through the isooctane membrane. The transport behavior suggested that the electrostatic interaction between the phosphoric part of the nucleotides and the cationic moiety of the ammonium salts permitted the transport of the nucleotides. The factors affecting the transport efficiency were found to be the carrier concentration in the liquid membrane phase and the salt concentration in the aqueous feed solution. The pH in the feed phase, which dominates the dissociation behavior of the phosphoric part of the nucleotides, also influenced the permeability. Adenosine mono-phosphate(AMP) could be separated from di- and tri-phosphates (ADP and ATP) by using the bulk liquid membrane system by adjusting the pH, the salt concentration and the carrier concentration in each phase.

Original languageEnglish
Pages (from-to)81-89
Number of pages9
JournalSolvent Extraction Research and Development
Publication statusPublished - Dec 1 2002

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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