Abstract
We developed a highly sensitive HTS SQUID system for liquid-phase detection of biological targets using Brownian relaxation of magnetic markers. The SQUID was made of a ramp-edge Josephson junction using RE123-based multilayer process, and it showed a flux noise of 7.5 micro flux-quantam/Hz1/2 in the white noise region. The 1/f noise was 14 micro flux-quantam/Hz1/2 at f = 1 Hz when the SQUID was operated with AC bias mode. Using the SQUID system, we detected a biological target called biotin. In the experiment, biotins were fixed to a large polymer bead with a diameter of 3.3 micron meter. Streptavidin-coated magnetic markers were used for the detection. The bound and unbound (free) markers were magnetically distinguished using the difference in their Brownian relaxation time, i.e., without using the washing process to separate them. The minimum detectable number of biotins was as low as 10 4, corresponding to a sensitivity of 4.8×10-19 mol/ml. This result indicates a high sensitivity of the developed method.
| Original language | English |
|---|---|
| Title of host publication | 2013 IEEE 14th InternationalSuperconductive Electronics Conference, ISEC 2013 |
| DOIs | |
| Publication status | Published - Oct 15 2013 |
| Event | 2013 IEEE 14th InternationalSuperconductive Electronics Conference, ISEC 2013 - Cambridge, MA, United States Duration: Jul 7 2013 → Jul 11 2013 |
Other
| Other | 2013 IEEE 14th InternationalSuperconductive Electronics Conference, ISEC 2013 |
|---|---|
| Country | United States |
| City | Cambridge, MA |
| Period | 7/7/13 → 7/11/13 |
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All Science Journal Classification (ASJC) codes
- Electrical and Electronic Engineering
Cite this
Liquid-phase detection of biological targets with magnetic markers and high Tc SQUID. / Uchida, S.; Higuchi, Y.; Ueoka, Y.; Yoshida, Takashi; Enpuku, Keiji; Adachi, S.; Tanabe, K.; Tsukamoto, A.
2013 IEEE 14th InternationalSuperconductive Electronics Conference, ISEC 2013. 2013. 6604304.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Liquid-phase detection of biological targets with magnetic markers and high Tc SQUID
AU - Uchida, S.
AU - Higuchi, Y.
AU - Ueoka, Y.
AU - Yoshida, Takashi
AU - Enpuku, Keiji
AU - Adachi, S.
AU - Tanabe, K.
AU - Tsukamoto, A.
PY - 2013/10/15
Y1 - 2013/10/15
N2 - We developed a highly sensitive HTS SQUID system for liquid-phase detection of biological targets using Brownian relaxation of magnetic markers. The SQUID was made of a ramp-edge Josephson junction using RE123-based multilayer process, and it showed a flux noise of 7.5 micro flux-quantam/Hz1/2 in the white noise region. The 1/f noise was 14 micro flux-quantam/Hz1/2 at f = 1 Hz when the SQUID was operated with AC bias mode. Using the SQUID system, we detected a biological target called biotin. In the experiment, biotins were fixed to a large polymer bead with a diameter of 3.3 micron meter. Streptavidin-coated magnetic markers were used for the detection. The bound and unbound (free) markers were magnetically distinguished using the difference in their Brownian relaxation time, i.e., without using the washing process to separate them. The minimum detectable number of biotins was as low as 10 4, corresponding to a sensitivity of 4.8×10-19 mol/ml. This result indicates a high sensitivity of the developed method.
AB - We developed a highly sensitive HTS SQUID system for liquid-phase detection of biological targets using Brownian relaxation of magnetic markers. The SQUID was made of a ramp-edge Josephson junction using RE123-based multilayer process, and it showed a flux noise of 7.5 micro flux-quantam/Hz1/2 in the white noise region. The 1/f noise was 14 micro flux-quantam/Hz1/2 at f = 1 Hz when the SQUID was operated with AC bias mode. Using the SQUID system, we detected a biological target called biotin. In the experiment, biotins were fixed to a large polymer bead with a diameter of 3.3 micron meter. Streptavidin-coated magnetic markers were used for the detection. The bound and unbound (free) markers were magnetically distinguished using the difference in their Brownian relaxation time, i.e., without using the washing process to separate them. The minimum detectable number of biotins was as low as 10 4, corresponding to a sensitivity of 4.8×10-19 mol/ml. This result indicates a high sensitivity of the developed method.
UR - http://www.scopus.com/inward/record.url?scp=84885196800&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84885196800&partnerID=8YFLogxK
U2 - 10.1109/ISEC.2013.6604304
DO - 10.1109/ISEC.2013.6604304
M3 - Conference contribution
SN - 9781467363716
BT - 2013 IEEE 14th InternationalSuperconductive Electronics Conference, ISEC 2013
ER -