Liquid-phase detection of biomaterial using magnetic markers

Yoshitsugu Takafuji, Kohta Irie, Takashi Yoshida, Keiji Enpuku

Research output: Contribution to journalArticle

Abstract

We have been developing a liquid-phase immunoassay using magnetic markers and a superconducting quantum interference device (SQUID). In this method, bound and free markers are magnetically differentiated by using the Brownian relaxation of the free markers. Hence, the time consuming washing process that is needed in the conventional optical method for Bound/Free (B/F) separation can be eliminated. Using the method, we demonstrated a detection of C-reactive protein (CRP). We obtained good correlation between the detected signal flux and the concentration of CRP. The minimum detectable concentration was 1 pg/mL. Since the present sensitivity is limited by the blank signal caused by the aggregation of free markers, much improvement will be possible if this problem is solved.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalResearch Reports on Information Science and Electrical Engineering of Kyushu University
Volume23
Issue number1
Publication statusPublished - Jan 2018

Fingerprint

Biomaterials
Proteins
SQUIDs
Liquids
Washing
Agglomeration
Fluxes

All Science Journal Classification (ASJC) codes

  • Computer Science(all)
  • Electrical and Electronic Engineering

Cite this

Liquid-phase detection of biomaterial using magnetic markers. / Takafuji, Yoshitsugu; Irie, Kohta; Yoshida, Takashi; Enpuku, Keiji.

In: Research Reports on Information Science and Electrical Engineering of Kyushu University, Vol. 23, No. 1, 01.2018, p. 1-7.

Research output: Contribution to journalArticle

@article{02133ba1bccf4b5c933c68fe98aaf8a3,
title = "Liquid-phase detection of biomaterial using magnetic markers",
abstract = "We have been developing a liquid-phase immunoassay using magnetic markers and a superconducting quantum interference device (SQUID). In this method, bound and free markers are magnetically differentiated by using the Brownian relaxation of the free markers. Hence, the time consuming washing process that is needed in the conventional optical method for Bound/Free (B/F) separation can be eliminated. Using the method, we demonstrated a detection of C-reactive protein (CRP). We obtained good correlation between the detected signal flux and the concentration of CRP. The minimum detectable concentration was 1 pg/mL. Since the present sensitivity is limited by the blank signal caused by the aggregation of free markers, much improvement will be possible if this problem is solved.",
author = "Yoshitsugu Takafuji and Kohta Irie and Takashi Yoshida and Keiji Enpuku",
year = "2018",
month = "1",
language = "English",
volume = "23",
pages = "1--7",
journal = "Research Reports on Information Science and Electrical Engineering of Kyushu University",
issn = "1342-3819",
publisher = "Kyushu University, Faculty of Science",
number = "1",

}

TY - JOUR

T1 - Liquid-phase detection of biomaterial using magnetic markers

AU - Takafuji, Yoshitsugu

AU - Irie, Kohta

AU - Yoshida, Takashi

AU - Enpuku, Keiji

PY - 2018/1

Y1 - 2018/1

N2 - We have been developing a liquid-phase immunoassay using magnetic markers and a superconducting quantum interference device (SQUID). In this method, bound and free markers are magnetically differentiated by using the Brownian relaxation of the free markers. Hence, the time consuming washing process that is needed in the conventional optical method for Bound/Free (B/F) separation can be eliminated. Using the method, we demonstrated a detection of C-reactive protein (CRP). We obtained good correlation between the detected signal flux and the concentration of CRP. The minimum detectable concentration was 1 pg/mL. Since the present sensitivity is limited by the blank signal caused by the aggregation of free markers, much improvement will be possible if this problem is solved.

AB - We have been developing a liquid-phase immunoassay using magnetic markers and a superconducting quantum interference device (SQUID). In this method, bound and free markers are magnetically differentiated by using the Brownian relaxation of the free markers. Hence, the time consuming washing process that is needed in the conventional optical method for Bound/Free (B/F) separation can be eliminated. Using the method, we demonstrated a detection of C-reactive protein (CRP). We obtained good correlation between the detected signal flux and the concentration of CRP. The minimum detectable concentration was 1 pg/mL. Since the present sensitivity is limited by the blank signal caused by the aggregation of free markers, much improvement will be possible if this problem is solved.

UR - http://www.scopus.com/inward/record.url?scp=85043582094&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85043582094&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:85043582094

VL - 23

SP - 1

EP - 7

JO - Research Reports on Information Science and Electrical Engineering of Kyushu University

JF - Research Reports on Information Science and Electrical Engineering of Kyushu University

SN - 1342-3819

IS - 1

ER -