Liquid-phase immunoassay utilizing binding reactions between magnetic markers and targets in the presence of a magnetic field

Keiji Enpuku, Yuta Ueoka, Tatsuto Sakakibara, Masakazu Ura, Takashi Yoshida, Takako Mizoguchi, Akihiko Kandori

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16 Citations (Scopus)

Abstract

We developed a new and improved method for the liquid-phase detection of biological targets using magnetic markers. Unlike conventional studies, we performed a binding reaction between markers and targets in the presence of a magnetic field. This field acts to prevent the Brownian rotation of markers during the reaction. In this case, markers are bound to the targets with their magnetic moments (m's) aligned, which is in contrast to the conventional case where m's are randomly oriented after the reaction. As a result, we could obtain much larger signals from the bound markers without increasing the blank signal from the free markers.

Original languageEnglish
Article number97001
JournalApplied Physics Express
Volume7
Issue number9
DOIs
Publication statusPublished - Sep 1 2014

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All Science Journal Classification (ASJC) codes

  • Engineering(all)
  • Physics and Astronomy(all)

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