Liquid-phase immunoassay utilizing binding reactions between magnetic markers and targets in the presence of a magnetic field

Keiji Enpuku; Yuta Ueoka; Tatsuto Sakakibara; Masakazu Ura; Takashi Yoshida; Takako Mizoguchi; Akihiko Kandori

doi:10.7567/APEX.7.097001

Liquid-phase immunoassay utilizing binding reactions between magnetic markers and targets in the presence of a magnetic field

Keiji Enpuku, Yuta Ueoka, Tatsuto Sakakibara, Masakazu Ura, Takashi Yoshida, Takako Mizoguchi, Akihiko Kandori

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

We developed a new and improved method for the liquid-phase detection of biological targets using magnetic markers. Unlike conventional studies, we performed a binding reaction between markers and targets in the presence of a magnetic field. This field acts to prevent the Brownian rotation of markers during the reaction. In this case, markers are bound to the targets with their magnetic moments (m's) aligned, which is in contrast to the conventional case where m's are randomly oriented after the reaction. As a result, we could obtain much larger signals from the bound markers without increasing the blank signal from the free markers.

Original language	English
Article number	97001
Journal	Applied Physics Express
Volume	7
Issue number	9
DOIs	https://doi.org/10.7567/APEX.7.097001
Publication status	Published - Sept 1 2014

All Science Journal Classification (ASJC) codes

Engineering(all)
Physics and Astronomy(all)

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abstract = "We developed a new and improved method for the liquid-phase detection of biological targets using magnetic markers. Unlike conventional studies, we performed a binding reaction between markers and targets in the presence of a magnetic field. This field acts to prevent the Brownian rotation of markers during the reaction. In this case, markers are bound to the targets with their magnetic moments (m's) aligned, which is in contrast to the conventional case where m's are randomly oriented after the reaction. As a result, we could obtain much larger signals from the bound markers without increasing the blank signal from the free markers.",

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AU - Enpuku, Keiji

AU - Ueoka, Yuta

AU - Sakakibara, Tatsuto

AU - Ura, Masakazu

AU - Yoshida, Takashi

AU - Mizoguchi, Takako

AU - Kandori, Akihiko

PY - 2014/9/1

Y1 - 2014/9/1

N2 - We developed a new and improved method for the liquid-phase detection of biological targets using magnetic markers. Unlike conventional studies, we performed a binding reaction between markers and targets in the presence of a magnetic field. This field acts to prevent the Brownian rotation of markers during the reaction. In this case, markers are bound to the targets with their magnetic moments (m's) aligned, which is in contrast to the conventional case where m's are randomly oriented after the reaction. As a result, we could obtain much larger signals from the bound markers without increasing the blank signal from the free markers.

AB - We developed a new and improved method for the liquid-phase detection of biological targets using magnetic markers. Unlike conventional studies, we performed a binding reaction between markers and targets in the presence of a magnetic field. This field acts to prevent the Brownian rotation of markers during the reaction. In this case, markers are bound to the targets with their magnetic moments (m's) aligned, which is in contrast to the conventional case where m's are randomly oriented after the reaction. As a result, we could obtain much larger signals from the bound markers without increasing the blank signal from the free markers.

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Liquid-phase immunoassay utilizing binding reactions between magnetic markers and targets in the presence of a magnetic field

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All Science Journal Classification (ASJC) codes

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