Lithium ions inhibit function of lowbut not high-affinity muscarinic receptors of murine neuroblastoma cells (clone N1E-115)

Shigenobu Kanba, Michael Pfenning, Elliott Richelson

Research output: Contribution to journalArticle

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Abstract

Murine neuroblastoma cells (clone N1E-115) possess both high- and low-affinity muscarinic receptors. The low-affinity muscarinic receptor, when stimulated, initiates the formation of cyclic GMP by activating the enzyme guanylate cyclase; whereas stimulation of the high-affinity receptor inhibits prostaglanding E1-mediated cyclic AMP formation by inhibiting the enzyme adenylate cyclase. We have reported that lithium ion (Li+) inhibits cyclic GMP formation mediated by the muscarinic receptor agonist, carbachol. in a concentration-dependent manner and that neither ammonium nor sodium ions have such an effect. We extended this study to show that Li+ was an apparently noncompetitive inhibitor of the low-affinity muscarinic receptor with an IC50 (±SEM)=13.6±0.8 mM. In addition, Li+ with a similar IC50 inhibited the cyclic GMP response in intact cells to sodium azide, which is thought to stimulate guanylate cyclase directly. Moreover, though Li+ was found to have a slight inhibitory effect on prostaglandin E1-stimulated cyclic AMP formation (15% inhibition at 10 mM), it had no effect on the function of the high-affinity muscarinic receptor in intact murine neuroblastoma cells.

Original languageEnglish
Pages (from-to)413-416
Number of pages4
JournalPsychopharmacology
Volume86
Issue number4
DOIs
Publication statusPublished - Aug 1 1985
Externally publishedYes

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Muscarinic Receptors
Neuroblastoma
Lithium
Clone Cells
Ions
Cyclic GMP
Guanylate Cyclase
Cyclic AMP
Inhibitory Concentration 50
Muscarinic Agonists
Sodium Azide
Alprostadil
Carbachol
Enzymes
Ammonium Compounds
Adenylyl Cyclases
Sodium

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Lithium ions inhibit function of lowbut not high-affinity muscarinic receptors of murine neuroblastoma cells (clone N1E-115). / Kanba, Shigenobu; Pfenning, Michael; Richelson, Elliott.

In: Psychopharmacology, Vol. 86, No. 4, 01.08.1985, p. 413-416.

Research output: Contribution to journalArticle

Kanba, Shigenobu ; Pfenning, Michael ; Richelson, Elliott. / Lithium ions inhibit function of lowbut not high-affinity muscarinic receptors of murine neuroblastoma cells (clone N1E-115). In: Psychopharmacology. 1985 ; Vol. 86, No. 4. pp. 413-416.
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