TY - JOUR
T1 - Live-Cell Imaging of DNA Methylation Based on Synthetic-Molecule/Protein Hybrid Probe
AU - Kumar, Naresh
AU - Hori, Yuichiro
AU - Kikuchi, Kazuya
N1 - Funding Information:
This research on the hybrid probe was supported by JSPS KAKENHI (Ggrant Numbers: JP25220207, JP16H00768, JP15 K12754, JP16 K13099 to K. K.; JP16F16331 to K. K. and N. K.; and JP26282215, JP17H02210, JP16 K13088, JP17H06005, JP16H01428 “Resonance Bio” to Y. H.), by AMED-CREST, and by SICORP from JST.
Publisher Copyright:
© 2018 The Chemical Society of Japan & Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2018/12
Y1 - 2018/12
N2 - The epigenetic modification of DNA involves the conversion of cytosine to 5-methylcytosine, also known as DNA methylation. DNA methylation is important in modulating gene expression and thus, regulating genome and cellular functions. Recent studies have shown that aberrations in DNA methylation are associated with various epigenetic disorders or diseases including cancer. This stimulates great interest in the development of methods that can detect and visualize DNA methylation. For instance, fluorescent proteins (FPs) in conjugation with methyl-CpG-binding domain (MBD) have been employed for live-cell imaging of DNA methylation. However, the FP-based approach showed fluorescence signals for both the DNA-bound and -unbound states and thus differentiation between these states is difficult. Synthetic-molecule/protein hybrid probes can provide an alternative to overcome this restriction. In this article, we discuss the synthetic-molecule/protein hybrid probe that we developed recently for live-cell imaging of DNA methylation, which exhibited fluorescence enhancement only after binding to methylated DNA.
AB - The epigenetic modification of DNA involves the conversion of cytosine to 5-methylcytosine, also known as DNA methylation. DNA methylation is important in modulating gene expression and thus, regulating genome and cellular functions. Recent studies have shown that aberrations in DNA methylation are associated with various epigenetic disorders or diseases including cancer. This stimulates great interest in the development of methods that can detect and visualize DNA methylation. For instance, fluorescent proteins (FPs) in conjugation with methyl-CpG-binding domain (MBD) have been employed for live-cell imaging of DNA methylation. However, the FP-based approach showed fluorescence signals for both the DNA-bound and -unbound states and thus differentiation between these states is difficult. Synthetic-molecule/protein hybrid probes can provide an alternative to overcome this restriction. In this article, we discuss the synthetic-molecule/protein hybrid probe that we developed recently for live-cell imaging of DNA methylation, which exhibited fluorescence enhancement only after binding to methylated DNA.
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U2 - 10.1002/tcr.201800039
DO - 10.1002/tcr.201800039
M3 - Review article
C2 - 29863802
AN - SCOPUS:85058711128
SN - 1527-8999
VL - 18
SP - 1672
EP - 1680
JO - Chemical Record
JF - Chemical Record
IS - 12
ER -
