Live cells-based cytotoxic sensorchip fabricated in a microfluidic system

Ken Ichi Wada, Akiyoshi Taniguchi, Jun Kobayashi, Masayuki Yamato, Teruo Okano

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

We report here a live cells-based sensorchip fabricated in microfluidic channels in which several laminar flows were achieved. In addition, we established a cytotoxic sensor cell line, which was transfected with a green fluorescence protein (GFP) plasmid derived from the heat shock protein 70B′ (HSP70B′) promoter, which is induced by cytotoxic reagents. The fluorescence in the sensor cells increased in a CdCl2 dose-dependent manner in the microfluidic channels. In this system, cytotoxic reagents can be quantitatively detected in a quick, sensitive and high-throughput manner. The combination of sensor cells and microfluidic systems will provide an important basis for the development of micro-total analysis systems (μ-TAS) technology, and can be applied to toxicology, environmental assessment and drug screening.

Original languageEnglish
Pages (from-to)1513-1517
Number of pages5
JournalBiotechnology and Bioengineering
Volume99
Issue number6
DOIs
Publication statusPublished - Apr 15 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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