@article{9324cee3decd4053baa74aedebf676f8,
title = "Live single cell mass spectrometry reveals cancer-specific metabolic profiles of circulating tumor cells",
abstract = "Recently, there has been increased attention on the analysis of circulating tumor cells (CTCs), also known as liquid biopsy, owing to its potential benefits in cancer diagnosis and treatment. Circulating tumor cells are released from primary tumor lesions into the blood stream and eventually metastasize to distant body organs. However, a major hurdle with CTC analysis is their natural scarcity. Existing methods lack sensitivity, specificity, or reproducibility required in CTC characterization and detection. Here, we report untargeted molecular profiling of single CTCs obtained from gastric cancer and colorectal cancer patients, using live single cell mass spectrometry integrated with microfluidics-based cell enrichment techniques. Using this approach, we showed the difference in the metabolomic profile between CTCs originating from different cancer groups. Moreover, potential biomarkers were putatively annotated to be specific to each cancer type.",
author = "Yasmine Abouleila and Kaoru Onidani and Ahmed Ali and Hirokazu Shoji and Takayuki Kawai and Lim, {Chwee Teck} and Vipin Kumar and Shinobu Okaya and Ken Kato and Eiso Hiyama and Toshio Yanagida and Tsutomu Masujima and Yoshihiro Shimizu and Kazufumi Honda",
note = "Funding Information: The authors thank Dr. Sami Emara, Dr. Walaa Zarad, and Dr. Mohammed Ashraf for critical discussion in the early phase of this project. This study was supported by grants from: RIKEN Junior Research Associate Program, RIKEN Single Cell Research Project, the Project for Cancer Research and Therapeutic Evolution (P-CREATE) from AMED (18cm0106403 h0003, to KH), AMED CREST from AMED (18gm0710010h0105 to KH), a Grant-in Aid for Scientific Research (B), and a Challenging Exploratory Research grant from the MinistryofEducation,Culture,Sports,ScienceandTechnologyofJapan (to KH). Funding Information: RIKEN Junior Research Associate Program; RIKEN Single Cell Research Project; Project for Cancer Research and Therapeutic Evolution (P-CREATE) from AMED, Grant Award No. 18cm0106403 h0003; AMED CREST from AMED, Grant Award No. 18gm0710010h0105; Grant-in Aid for Scientific Research (B), and a Challenging Exploratory Research grant from the Ministry of Education, Culture, Sports, Science and Technology of Japan Publisher Copyright: {\textcopyright} 2018 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.",
year = "2019",
month = feb,
doi = "10.1111/cas.13915",
language = "English",
volume = "110",
pages = "697--706",
journal = "Cancer Science",
issn = "1347-9032",
publisher = "Wiley-Blackwell",
number = "2",
}
