Liver cell-specific peptides derived from the preS1 domain of human hepatitis B virus

Jeong Hun Kang, Riki Toita, Daisuke Asai, Tetsuji Yamaoka, Masaharu Murata

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    The envelope of human hepatitis B virus (HBV) consists of the large (L), middle (M), and small (S) surface proteins. The preS1 domain at the N terminus of the L-protein is essential for recognizing a target cell and for viral infectivity. In the present study, peptides derived from the preS1 domain (amino acid residues 2-19) were synthesized, and their binding affinities for human hepatocellular carcinoma (HCC) cells were determined. Non-myristoylated peptides showed much lower affinity for HepG2 cells than myristoylated peptides. Although all peptides showed significantly higher affinities for two human HCC cell lines (HepG2 and HuH-7) compared with other cell lines (HeLa, B16, NMuLi, and NIH 3T3), a modified peptide exhibited the highest affinity for HCC cell lines. These results suggest that the modified peptide can target liver cells.

    Original languageEnglish
    Pages (from-to)20-23
    Number of pages4
    JournalJournal of Virological Methods
    Volume201
    DOIs
    Publication statusPublished - Jun 1 2014

    All Science Journal Classification (ASJC) codes

    • Virology

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