Liver cell specific targeting by the preS1 domain of hepatitis B virus surface antigen displayed on protein nanocages

Masaharu Murata, Sayoko Narahara, Kaori Umezaki, Riki Toita, Shigekazu Tabata, Jing Shu Piao, Kana Abe, Jeong Hun Kang, Kenoki Ohuchida, Lin Cui, Makoto Hashizume

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Protein nanocages are self-organized complexes of oligomers whose three-dimensional architecture can been determined in detail. These structures possess nanoscale inner cavities into which a variety of molecules, including therapeutic or diagnostic agents, can be encapsulated. These properties yield these particles suitable for a new class of drug delivery carrier, or as a bioimaging reagent that might respond to biochemical signals in many different cellular processes. We report here the design, synthesis, and biological characterization of a hepatocyte-specific nanocage carrying small heat-shock protein. These nanoscale protein cages, with a targeting peptide composed of a preS1 derivative from the hepatitis B virus on their surfaces, were prepared by genetic engineering techniques. PreS1-carrying nanocages showed lower cytotoxicity and significantly higher specificity for human hepatocyte cell lines than other cell lines in vitro. These results suggested that small heat-shock protein-based nanocages present great potential for the development of effective targeted delivery of various agents to specific cells.

Original languageEnglish
Pages (from-to)4353-4362
Number of pages10
JournalInternational journal of nanomedicine
Volume7
DOIs
Publication statusPublished - Dec 5 2012

Fingerprint

Small Heat-Shock Proteins
Surface Antigens
Antigens
Hepatitis B Surface Antigens
Viruses
Hepatitis B virus
Liver
Hepatocytes
Cells
Proteins
Genetic Techniques
Genetic engineering
Cell Line
Drug Carriers
Genetic Engineering
Cytotoxicity
Drug delivery
Oligomers
Derivatives
Peptides

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

Cite this

Liver cell specific targeting by the preS1 domain of hepatitis B virus surface antigen displayed on protein nanocages. / Murata, Masaharu; Narahara, Sayoko; Umezaki, Kaori; Toita, Riki; Tabata, Shigekazu; Piao, Jing Shu; Abe, Kana; Kang, Jeong Hun; Ohuchida, Kenoki; Cui, Lin; Hashizume, Makoto.

In: International journal of nanomedicine, Vol. 7, 05.12.2012, p. 4353-4362.

Research output: Contribution to journalArticle

Murata, M, Narahara, S, Umezaki, K, Toita, R, Tabata, S, Piao, JS, Abe, K, Kang, JH, Ohuchida, K, Cui, L & Hashizume, M 2012, 'Liver cell specific targeting by the preS1 domain of hepatitis B virus surface antigen displayed on protein nanocages', International journal of nanomedicine, vol. 7, pp. 4353-4362. https://doi.org/10.2147/IJN.S31365
Murata, Masaharu ; Narahara, Sayoko ; Umezaki, Kaori ; Toita, Riki ; Tabata, Shigekazu ; Piao, Jing Shu ; Abe, Kana ; Kang, Jeong Hun ; Ohuchida, Kenoki ; Cui, Lin ; Hashizume, Makoto. / Liver cell specific targeting by the preS1 domain of hepatitis B virus surface antigen displayed on protein nanocages. In: International journal of nanomedicine. 2012 ; Vol. 7. pp. 4353-4362.
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