Liver receptor homologue-1 (LRH-1) activates the promoter of brain aromatase (cyp19a2) in a teleost fish, the medaka, Oryzias latipes

Yuki Ohmuro-Matsuyama, Kataaki Okubo, Masaru Matsuda, Shigeho Ijiri, Deshou Wang, Guijun Guan, Taiga Suzuki, Makoto Matsuyama, Ken Ichiro Morohashi, Yoshitaka Nagahama

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11 Citations (Scopus)

Abstract

The medaka, Oryzias latipes, like other fish, have two distinct aromatase genes, the ovarian (cyp19a1) and brain (cyp19a2) forms. We previously reported that Ad4BP/SF-1, a member of the NR5A subfamily, plays an important role in the regulation of cyp19a1 expression in medaka ovarian follicles during vitellogenesis. In the present study, we investigated whether liver receptor homologue-1 (LRH-1), another NR5A subfamily member, is involved in the regulation of cyp19a2 expression in the medaka brain. In situ hybridization analysis revealed that LRH-1 was expressed in the hypothalamus, where it colocalized with aromatase (cyp19a2). We then showed by transient transfection assays that LRH-1 was able to increase expression of a cyp19a2 reporter gene in various mammalian cell lines, and that mutation of a putative LRH-1 binding site within the cyp19a2 promoter abolished this effect. Taken together, these findings suggest that LRH-1 plays a role in regulating cyp19a2 expression in the medaka brain. This is the first to demonstrate in vitro the activation of brain aromatase by LRH-1 in the vertebrate brain.

Original languageEnglish
Pages (from-to)1065-1071
Number of pages7
JournalMolecular Reproduction and Development
Volume74
Issue number9
DOIs
Publication statusPublished - Sep 1 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology
  • Cell Biology

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    Ohmuro-Matsuyama, Y., Okubo, K., Matsuda, M., Ijiri, S., Wang, D., Guan, G., Suzuki, T., Matsuyama, M., Morohashi, K. I., & Nagahama, Y. (2007). Liver receptor homologue-1 (LRH-1) activates the promoter of brain aromatase (cyp19a2) in a teleost fish, the medaka, Oryzias latipes. Molecular Reproduction and Development, 74(9), 1065-1071. https://doi.org/10.1002/mrd.20497