Liver regenerative therapy using glycoside-modified bone marrow

Hirohiko Ise, Masafumi Takahashi, Uichi Ikeda

Research output: Contribution to journalReview article

Abstract

Recent studies have reported that bone marrow cells (BMCs) have the ability to generate functional hepatocytes. However, the efficiency with which BMC transplantation generates functional hepatocytes is rather low. We assumed that if BMCs accumulated directly in liver, the functional BMC-derived hepatocytes should increase more efficiently. An attempt was made to increase the accumulation of BMCs directly in liver through the interaction between hepatic asialoglycoprotein receptor (ASGPR) and galactose-exposing BMCs. Galactose-exposing BMCs that expressed green fluorescent protein (GFP) were injected into Long-Evans-Cinnamon (LEC) rats, a Wilson's disease (WD) model, via the tail vein. The WD is an autosomal-recessive disorder characterized by impaired biliary copper excretion and copper toxicosis, all due to mutations in the atp7b gene. At 5 months after transplantation, GFP-expressing hepatocyte nodules accounted for 2.4% of total liver mass, and the normal ceruloplasmin was detectable in the sera of these LEC rats. These findings suggest that the functional BMC-derived hepatocytes can be generated and the new genes derived from BMCs, such as ATP7B and GFP, can be transferred to LEC rats by the direct accumulation of BMCs in liver without hematopoietic reconstitution in need of preparative lethal irradiation.

Original languageEnglish
Pages (from-to)2229-2236
Number of pages8
JournalNippon rinsho. Japanese journal of clinical medicine
Volume63
Issue number12
Publication statusPublished - Jan 1 2005
Externally publishedYes

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Glycosides
Bone Marrow Cells
Bone Marrow
Liver
Inbred LEC Rats
Hepatocytes
Green Fluorescent Proteins
Hepatolenticular Degeneration
Therapeutics
Galactose
Copper
Asialoglycoprotein Receptor
Ceruloplasmin
Bone Marrow Transplantation
Genes
Tail
Veins
Transplantation
Mutation
Serum

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Liver regenerative therapy using glycoside-modified bone marrow. / Ise, Hirohiko; Takahashi, Masafumi; Ikeda, Uichi.

In: Nippon rinsho. Japanese journal of clinical medicine, Vol. 63, No. 12, 01.01.2005, p. 2229-2236.

Research output: Contribution to journalReview article

Ise, Hirohiko ; Takahashi, Masafumi ; Ikeda, Uichi. / Liver regenerative therapy using glycoside-modified bone marrow. In: Nippon rinsho. Japanese journal of clinical medicine. 2005 ; Vol. 63, No. 12. pp. 2229-2236.
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