Living biointerfaces based on non-pathogenic bacteria to direct cell differentiation

Aleixandre Rodrigo-Navarro, Patricia Rico, Anas Saadeddin, Andres J. Garcia, Manuel Salmeron-Sanchez

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9 Citations (Scopus)

Abstract

Genetically modified Lactococcus lactis, non-pathogenic bacteria expressing the FNIII 7-10 fibronectin fragment as a protein membrane have been used to create a living biointerface between synthetic materials and mammalian cells. This FNIII 7-10 fragment comprises the RGD and PHSRN sequences of fibronectin to bind 5 21 integrins and triggers signalling for cell adhesion, spreading and differentiation. We used L. lactis strain to colonize material surfaces and produce stable biofilms presenting the FNIII 7-10 fragment readily available to cells. Biofilm density is easily tunable and remains stable for several days. Murine C2C12 myoblasts seeded over mature biofilms undergo bipolar alignment and form differentiated myotubes, a process triggered by the FNIII 7-10 fragment. This biointerface based on living bacteria can be further modified to express any desired biochemical signal, establishing a new paradigm in biomaterial surface functionalisation for biomedical applications.

Original languageEnglish
Article number5849
JournalScientific reports
Volume4
DOIs
Publication statusPublished - Jul 28 2014

All Science Journal Classification (ASJC) codes

  • General

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    Rodrigo-Navarro, A., Rico, P., Saadeddin, A., Garcia, A. J., & Salmeron-Sanchez, M. (2014). Living biointerfaces based on non-pathogenic bacteria to direct cell differentiation. Scientific reports, 4, [5849]. https://doi.org/10.1038/srep05849