Local OPG gene transfer to periodontal tissue inhibits orthodontic tooth movement

H. Kanzaki, M. Chiba, Ichiro Takahashi, Naoto Haruyama, M. Nishimura, H. Mitani

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Previously, we discovered that RANKL expression is induced in compressed periodontal ligament cells, and that this promotes osteoclastogenesis on the compression side in orthodontic tooth movement. We hypothesized that local OPG gene transfer to the periodontium would neutralize the RANKL activity induced by mechanical compressive force, thereby inhibiting osteoclastogenesis and diminishing tooth movement. The upper first molars of six-week-old male Wistar rats were moved palatally by means of a fixed-orthodontic wire. A mouse OPG expression plasmid [pcDNA3.1(+)-mOPG] was constructed, and the production of functional OPG protein was confirmed in vitro. The inactivated HVJ envelope vector containing pcDNA3.1(+)-mOPG or PBS was injected periodically into the palatal periodontal tissue of upper first molars. When this local OPG gene transfer was performed, OPG production was induced, and osteoclastogenesis was inhibited. Local OPG gene transfer significantly diminished tooth movement. In this study, we report that OPG gene transfer to periodontal tissue inhibited RANKL-mediated osteoclastogenesis and inhibited experimental tooth movement.

Original languageEnglish
Pages (from-to)920-925
Number of pages6
JournalJournal of Dental Research
Volume83
Issue number12
DOIs
Publication statusPublished - Jan 1 2004
Externally publishedYes

Fingerprint

Tooth Movement Techniques
Osteogenesis
Genes
Orthodontic Wires
Periodontium
Periodontal Ligament
Wistar Rats
Plasmids
Proteins

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Local OPG gene transfer to periodontal tissue inhibits orthodontic tooth movement. / Kanzaki, H.; Chiba, M.; Takahashi, Ichiro; Haruyama, Naoto; Nishimura, M.; Mitani, H.

In: Journal of Dental Research, Vol. 83, No. 12, 01.01.2004, p. 920-925.

Research output: Contribution to journalArticle

Kanzaki, H. ; Chiba, M. ; Takahashi, Ichiro ; Haruyama, Naoto ; Nishimura, M. ; Mitani, H. / Local OPG gene transfer to periodontal tissue inhibits orthodontic tooth movement. In: Journal of Dental Research. 2004 ; Vol. 83, No. 12. pp. 920-925.
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