Localization, accumulation, and antifungal activity of chitinases in rye (Secale cereale) seed

Toki Taira, Takeshi Yamagami, Yoichi Aso, Masatsune Ishiguro, Masanobu Ishihara

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

In order to understand a physiological role of chitinases in rye, the localization and accumulation of rye seed chitinase-a and -c (RSC-a and -c) in the seeds were studied by immunochemical methods. An antiserum specific to the chitin-binding domain (CB-domain), which is an N-terminal part of RSC-a, and an antiserum specific to the catalytic region of RSC-a and RSC-c were used. An immunoblot analysis detected both RSC-a and RSC-c in the endosperm of the rye seed. Immuno-histochemical staining indicated that RSC-a was localized in only the aleurone cells, whereas RSC-c existed at least in the starchy endosperm and was also likely to exist in the aleurone cells. It was found by ELISA and an immunoblot analysis that RSC-a and -c accumulated in the seed during the later stage of development. Both chitinases and the Cat-domain exhibited antifungal activity toward Trichoderma species, while the CB-domain did not. Observation of the inhibition of hyphal growth of the T. species suggests that the two chitinases acted in different ways.

Original languageEnglish
Pages (from-to)2710-2718
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume65
Issue number12
DOIs
Publication statusPublished - Dec 2001

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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