Localization and expression of phospholipases A2 in Trimeresurus flavoviridis (habu snake) venom gland

Tomohisa Ogawa; Hitoshi Onoue; Kazunori Nakagawa; Shintarou Nomura; Katsuo Sueishi; Shosaku Hattori; Hiroshi Kihara; Motonori Ohno

doi:10.1016/0041-0101(95)00099-2

Localization and expression of phospholipases A₂ in Trimeresurus flavoviridis (habu snake) venom gland

Tomohisa Ogawa, Hitoshi Onoue, Kazunori Nakagawa, Shintarou Nomura, Katsuo Sueishi, Shosaku Hattori, Hiroshi Kihara, Motonori Ohno

Pathobiology

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6 Citations (Scopus)

Abstract

The localization and expression profiles of phospholipases A₂ (PLA₂s) in Trimeresurus flavoviridis venom gland were studied by means of in situ hybridization and immunohistochemical techniques. Venom gland cells are tightly arrayed in a single layer along the inlet-like lumens in which venom proteins are stored. mRNAs for PLA₂s were detected at the high level in cytoplasm. Using the immunohistochemical technique with polyclonal antiAsp-49-PLA₂ antibody, Asp-49-PLA₂ and, possibly, its isozymes were detected in intracellular granules and in venom lumens. The intracellular granules containing PLA₂ proteins appear to be transferred from the nucleus towards the outer membrane facing the lumen, and then to be secreted.

Original language	English
Pages (from-to)	1645-1652
Number of pages	8
Journal	Toxicon
Volume	33
Issue number	12
DOIs	https://doi.org/10.1016/0041-0101(95)00099-2
Publication status	Published - Dec 1995

All Science Journal Classification (ASJC) codes

Toxicology

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10.1016/0041-0101(95)00099-2

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title = "Localization and expression of phospholipases A2 in Trimeresurus flavoviridis (habu snake) venom gland",

abstract = "The localization and expression profiles of phospholipases A2 (PLA2s) in Trimeresurus flavoviridis venom gland were studied by means of in situ hybridization and immunohistochemical techniques. Venom gland cells are tightly arrayed in a single layer along the inlet-like lumens in which venom proteins are stored. mRNAs for PLA2s were detected at the high level in cytoplasm. Using the immunohistochemical technique with polyclonal antiAsp-49-PLA2 antibody, Asp-49-PLA2 and, possibly, its isozymes were detected in intracellular granules and in venom lumens. The intracellular granules containing PLA2 proteins appear to be transferred from the nucleus towards the outer membrane facing the lumen, and then to be secreted.",

author = "Tomohisa Ogawa and Hitoshi Onoue and Kazunori Nakagawa and Shintarou Nomura and Katsuo Sueishi and Shosaku Hattori and Hiroshi Kihara and Motonori Ohno",

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T1 - Localization and expression of phospholipases A2 in Trimeresurus flavoviridis (habu snake) venom gland

AU - Ogawa, Tomohisa

AU - Onoue, Hitoshi

AU - Nakagawa, Kazunori

AU - Nomura, Shintarou

AU - Sueishi, Katsuo

AU - Hattori, Shosaku

AU - Kihara, Hiroshi

AU - Ohno, Motonori

PY - 1995/12

Y1 - 1995/12

N2 - The localization and expression profiles of phospholipases A2 (PLA2s) in Trimeresurus flavoviridis venom gland were studied by means of in situ hybridization and immunohistochemical techniques. Venom gland cells are tightly arrayed in a single layer along the inlet-like lumens in which venom proteins are stored. mRNAs for PLA2s were detected at the high level in cytoplasm. Using the immunohistochemical technique with polyclonal antiAsp-49-PLA2 antibody, Asp-49-PLA2 and, possibly, its isozymes were detected in intracellular granules and in venom lumens. The intracellular granules containing PLA2 proteins appear to be transferred from the nucleus towards the outer membrane facing the lumen, and then to be secreted.

AB - The localization and expression profiles of phospholipases A2 (PLA2s) in Trimeresurus flavoviridis venom gland were studied by means of in situ hybridization and immunohistochemical techniques. Venom gland cells are tightly arrayed in a single layer along the inlet-like lumens in which venom proteins are stored. mRNAs for PLA2s were detected at the high level in cytoplasm. Using the immunohistochemical technique with polyclonal antiAsp-49-PLA2 antibody, Asp-49-PLA2 and, possibly, its isozymes were detected in intracellular granules and in venom lumens. The intracellular granules containing PLA2 proteins appear to be transferred from the nucleus towards the outer membrane facing the lumen, and then to be secreted.

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Localization and expression of phospholipases A₂ in Trimeresurus flavoviridis (habu snake) venom gland

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