Localization and interaction of the biosynthetic proteins for the lantibiotic, nukacin ISK-1

Jun Ichi Nagao, Yuji Aso, Toshihiro Sashihara, Kouki Shioya, Asaho Adachi, Jiro Nakayama, Kenji Sonomoto

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Nukacin ISK-1 is a type-A(II) lantibiotic produced by Staphylococcus warneri ISK-1. In this study, we characterized NukM and NukT, which are predicted to be involved in modification of prepeptide (NukA) and cleavage of leader peptide and subsequent secretion respectively. Localization analysis of NukM and NukT in the wild-type strain indicated that both proteins were located at the cytoplasm membrane. Interestingly, NukM expressed heterologously in St. carnosus TM300 was also located at the cytoplasm membrane even in the absence of NukT. Yeast two-hybrid assay showed that a complex of at least two each of NukM and NukT was associated with NukA. In vitro interaction analysis by surface plasmon resonance biosensor further suggested that membrane-located NukM interacted with NukA. These results indicate that NukM and NukT form a membrane-located multimeric protein complex and that post-translational modification of nukacin ISK-1 would occur at the cytoplasm membrane.

Original languageEnglish
Pages (from-to)1341-1347
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume69
Issue number7
DOIs
Publication statusPublished - Sep 1 2005

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Bacteriocins
Recombinant Proteins
Membranes
Cytoplasm
Two-Hybrid System Techniques
Surface Plasmon Resonance
Surface plasmon resonance
Biosensing Techniques
Post Translational Protein Processing
Protein Sorting Signals
Staphylococcus
Biosensors
Yeast
Assays
Proteins
nukacin ISK-1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Localization and interaction of the biosynthetic proteins for the lantibiotic, nukacin ISK-1. / Nagao, Jun Ichi; Aso, Yuji; Sashihara, Toshihiro; Shioya, Kouki; Adachi, Asaho; Nakayama, Jiro; Sonomoto, Kenji.

In: Bioscience, Biotechnology and Biochemistry, Vol. 69, No. 7, 01.09.2005, p. 1341-1347.

Research output: Contribution to journalArticle

Nagao, Jun Ichi ; Aso, Yuji ; Sashihara, Toshihiro ; Shioya, Kouki ; Adachi, Asaho ; Nakayama, Jiro ; Sonomoto, Kenji. / Localization and interaction of the biosynthetic proteins for the lantibiotic, nukacin ISK-1. In: Bioscience, Biotechnology and Biochemistry. 2005 ; Vol. 69, No. 7. pp. 1341-1347.
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