TY - JOUR
T1 - Localization of a novel inositol 1,4,5-trisphosphate binding protein, p130 in rat brain
AU - Matsuda, Miho
AU - Kanematsu, Takashi
AU - Takeuchi, Hiroshi
AU - Kukita, Toshio
AU - Hirata, Masato
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan (to TK and MH), and by The Ryoichi Naito Foundation for Medical Research and the Fukuoka Cancer Society (to MH). We thank Drs. T. Kosaka and K. Nakamura (Department of Anatomy, Faculty of Medicine, Kyushu University) for advice in Histology.
PY - 1998/11/27
Y1 - 1998/11/27
N2 - We have isolated a novel inositol 1,4,5-trisphosphate binding protein with molecular mass of 130 kDa (p130), homologous to phospholipase C-δ1 in amino acid sequence but with no catalytic activity. Here we report the expression and localization of p130 at the mRNA level in rat brain. Northern blotting showed that gene expression encoding p130 was most abundant in brain. Brain localization of p130-mRNA using an in situ hybridization technique revealed that in the cerebellum, the mRNA was detected in the granular cell and Purkinje cell layers, and cerebellar nuclei. In the cerebrum, the mRNA was localized in hippocampal pyramidal cells, dentate granule cells and pyramidal and/or granule cells of the cerebral cortex. The brain localization of p130-mRNA was similar to that of the β-subtype of phospholipase C, indicating that p130 may be mainly involved in phospholipase Cβ-mediated signaling.
AB - We have isolated a novel inositol 1,4,5-trisphosphate binding protein with molecular mass of 130 kDa (p130), homologous to phospholipase C-δ1 in amino acid sequence but with no catalytic activity. Here we report the expression and localization of p130 at the mRNA level in rat brain. Northern blotting showed that gene expression encoding p130 was most abundant in brain. Brain localization of p130-mRNA using an in situ hybridization technique revealed that in the cerebellum, the mRNA was detected in the granular cell and Purkinje cell layers, and cerebellar nuclei. In the cerebrum, the mRNA was localized in hippocampal pyramidal cells, dentate granule cells and pyramidal and/or granule cells of the cerebral cortex. The brain localization of p130-mRNA was similar to that of the β-subtype of phospholipase C, indicating that p130 may be mainly involved in phospholipase Cβ-mediated signaling.
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U2 - 10.1016/S0304-3940(98)00810-6
DO - 10.1016/S0304-3940(98)00810-6
M3 - Article
C2 - 9865936
AN - SCOPUS:0032573616
SN - 0304-3940
VL - 257
SP - 97
EP - 100
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2
ER -