Localization of a novel inositol 1,4,5-trisphosphate binding protein, p130 in rat brain

Miho Matsuda, Takashi Kanematsu, Hiroshi Takeuchi, Toshio Kukita, Masato Hirata

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We have isolated a novel inositol 1,4,5-trisphosphate binding protein with molecular mass of 130 kDa (p130), homologous to phospholipase C-δ1 in amino acid sequence but with no catalytic activity. Here we report the expression and localization of p130 at the mRNA level in rat brain. Northern blotting showed that gene expression encoding p130 was most abundant in brain. Brain localization of p130-mRNA using an in situ hybridization technique revealed that in the cerebellum, the mRNA was detected in the granular cell and Purkinje cell layers, and cerebellar nuclei. In the cerebrum, the mRNA was localized in hippocampal pyramidal cells, dentate granule cells and pyramidal and/or granule cells of the cerebral cortex. The brain localization of p130-mRNA was similar to that of the β-subtype of phospholipase C, indicating that p130 may be mainly involved in phospholipase Cβ-mediated signaling.

Original languageEnglish
Pages (from-to)97-100
Number of pages4
JournalNeuroscience Letters
Volume257
Issue number2
DOIs
Publication statusPublished - Nov 27 1998

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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