Localization of a syntaxin isoform, syntaxin 2, to the acrosomal region of rodent spermatozoa

Kanotomi Katafuchi, Takayuki Mori, Kiyotaka Toshimori, Hiroshi Iida

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The acrosome reaction includes a membrane fusion event that is a prerequisite for sperm penetration through the zona pellucida and subsequent fertilization. Since SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins have been shown to be key players in membrane fusion during regulated exocytosis in nerve terminals and secretory cells, and since the acrosome reaction has some features in common with regulated exocytosis, we hypothesized that SNARE proteins might also regulate acrosomal exocytosis. RT-PCR analysis demonstrated the expression of SNARE proteins, three isoforms of syntaxin 2 (2A, 2B, and 2C) and syntaxin 4A, in rat testes. Immunoblot analysis with antisyntaxin 2 antibody showed that the protein was expressed in rodent spermatozoa, and that it was associated with membrane components of spermatozoa prepared by sucrose density gradient centrifugation. Confocal laser scanning microscopy with double immunolabeling revealed that syntaxin 2 was colocalized with acrin 1, a 90 kDa acrosomal protein, over the acrosomal region of spermatozoa but was not associated with the posterior half of head or tail. Localization of syntaxin 2 over the acrosomal region was supported by the finding that it was shed from sperm heads during an acrosome reaction induced by calcium ionophore A23187 in vitro. In view of the putative role of syntaxin proteins in other membrane fusion systems, these data suggest that syntaxin 2 may be involved in regulating the acrosomal reaction in rodent spermatozoa. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)375-383
Number of pages9
JournalMolecular Reproduction and Development
Volume57
Issue number4
DOIs
Publication statusPublished - 2000

Fingerprint

Syntaxin 1
Qa-SNARE Proteins
SNARE Proteins
Acrosome Reaction
Membrane Fusion
Spermatozoa
Rodentia
Exocytosis
Protein Isoforms
Sperm-Ovum Interactions
Sperm Head
Proteins
Density Gradient Centrifugation
Calcium Ionophores
Calcimycin
Fertilization
Information Systems
Confocal Microscopy
Sucrose
Tail

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Localization of a syntaxin isoform, syntaxin 2, to the acrosomal region of rodent spermatozoa. / Katafuchi, Kanotomi; Mori, Takayuki; Toshimori, Kiyotaka; Iida, Hiroshi.

In: Molecular Reproduction and Development, Vol. 57, No. 4, 2000, p. 375-383.

Research output: Contribution to journalArticle

Katafuchi, Kanotomi ; Mori, Takayuki ; Toshimori, Kiyotaka ; Iida, Hiroshi. / Localization of a syntaxin isoform, syntaxin 2, to the acrosomal region of rodent spermatozoa. In: Molecular Reproduction and Development. 2000 ; Vol. 57, No. 4. pp. 375-383.
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