Localization of an Aldo-Keto Reductase (AKR2E4) in the Silkworm Bombyx mori (Lepidoptera: Bombycidae)

K. Yamamoto, Y. Ozakiya, T. Uno

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The aldo-keto reductase AKR2E4 reduces 3-dehydroecdysone to ecdysone in the silkworm Bombyx mori L. In this study, a quantitative polymerase chain reaction analysis revealed that the level of AKR2E4 mRNA was higher in the testes than in other tissues, and a western immunoblot analysis revealed that the AKR2E4 content in the testes was stage-specific from the fifth larval instar to the pupal stage. Immunohistochemical analysis showed that the AKR2E4 protein was present in cyst cells associated with sperm cells and spermatocytes. These results indicate that AKR2E4 plays an important role in 3-dehydroecdysone conversion to ecdysone and spermatogenesis in silkworm testes.

Original languageEnglish
Article number94
JournalJournal of Insect Science
Volume17
Issue number5
DOIs
Publication statusPublished - Jan 1 2017

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Bombycidae
Bombyx mori
silkworms
testes
ecdysone
Lepidoptera
spermatocytes
spermatogenesis
quantitative polymerase chain reaction
instars
spermatozoa
cells
proteins

All Science Journal Classification (ASJC) codes

  • Insect Science

Cite this

Localization of an Aldo-Keto Reductase (AKR2E4) in the Silkworm Bombyx mori (Lepidoptera : Bombycidae). / Yamamoto, K.; Ozakiya, Y.; Uno, T.

In: Journal of Insect Science, Vol. 17, No. 5, 94, 01.01.2017.

Research output: Contribution to journalArticle

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