Localization of an endocannabinoid system in the hypophysial pars tuberalis and pars distalis of man

Shinobu Yasuo, Claudia Unfried, Mattias Kettner, Gerd Geisslinger, Horst Werner Korf

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The hypophysial pars tuberalis (PT) acts as an important interface between neuroendocrine brain centers (hypothalamus, pineal organ) and the pars distalis (PD) of the hypophysis. Recently, we have identified an endocannabinoid system in the PT of hamsters and provided evidence that 2-arachidonoylglycerol is a messenger molecule that appears to play an essential role in seasonal reproduction and prolactin release by acting on the cannabinoid receptors in the PD. We now demonstrate the enzymes involved in endocannabinoid synthesis and degradation, namely sn-1-selective diacylglycerol lipase α, N-acylphosphatidylethanolamine-specific phospholipase D, and monoacylglycerol lipase, in the PT of man by means of immunohistochemistry. High-performance liquid chromatography coupled with tandem mass spectrometry revealed 2-arachidonoylglycerol and other endocannabinoids in the human PT. Furthermore, we detected the expression of the cannabinoid receptor 1 (CB1), a primary receptor for endocannabinoids, in the PD. Double-immunofluorescence staining for CB1 and various hypophysial hormones or S-100, a marker for folliculostellate (FS) cells, revealed that CB1 immunoreactivity was mainly localized to corticotrophs and FS-cells. A limited number of lactotrophs and somatotrophs also showed CB1 immunoreactivity, which was however absent from gonadotrophs and thyrotrophs. Our data thus indicate that the human PT comprises an endocannabinoid system, and that corticotrophs and FS-cells are the main target cells for endocannabinoids. The functional significance of this newly discovered pathway remains to be elucidated in man; it might be related to the control of stress responses and/or reflect a remnant seasonal control of hypophysial hormonal secretion.

Original languageEnglish
Pages (from-to)273-281
Number of pages9
JournalCell and tissue research
Volume342
Issue number2
DOIs
Publication statusPublished - Nov 1 2010

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Endocannabinoids
Cannabinoid Receptors
Corticotrophs
Monoacylglycerol Lipases
Thyrotrophs
Lactotrophs
Somatotrophs
Gonadotrophs
Phospholipase D
Lipoprotein Lipase
Pituitary Gland
Tandem Mass Spectrometry
Cricetinae
Prolactin
Hypothalamus
Fluorescent Antibody Technique
Reproduction
Immunohistochemistry
High Pressure Liquid Chromatography
Hormones

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Localization of an endocannabinoid system in the hypophysial pars tuberalis and pars distalis of man. / Yasuo, Shinobu; Unfried, Claudia; Kettner, Mattias; Geisslinger, Gerd; Korf, Horst Werner.

In: Cell and tissue research, Vol. 342, No. 2, 01.11.2010, p. 273-281.

Research output: Contribution to journalArticle

Yasuo, Shinobu ; Unfried, Claudia ; Kettner, Mattias ; Geisslinger, Gerd ; Korf, Horst Werner. / Localization of an endocannabinoid system in the hypophysial pars tuberalis and pars distalis of man. In: Cell and tissue research. 2010 ; Vol. 342, No. 2. pp. 273-281.
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