Abstract
The subcellular localization of plant farnesyl diphosphate synthase (FPPS) was examined. Immunocytochemical staining using anti-FPPS1 antibody followed by electron microscopy showed that FPPS1 was localized to chloroplasts of rice mesophyll cells. Subcellular fractions from wheat leaves were examined by immunoblot analysis. FPPS was detected in the chloroplast fraction in wheat, and was protected from proteolysis following trypsin treatment of chloroplasts. FPPS was also detected in the chloroplast fraction of a dicot plant, tobacco.
| Original language | English |
|---|---|
| Pages (from-to) | 348-354 |
| Number of pages | 7 |
| Journal | Plant and Cell Physiology |
| Volume | 40 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jan 1 1999 |
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All Science Journal Classification (ASJC) codes
- Physiology
- Plant Science
- Cell Biology
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Localization of farnesyl diphosphate synthase in chloroplasts. / Sanmiya, Kazutsuka; Osamu, Ueno; Matsuoka, Makoto; Yamamoto, Naoki.
In: Plant and Cell Physiology, Vol. 40, No. 3, 01.01.1999, p. 348-354.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Localization of farnesyl diphosphate synthase in chloroplasts
AU - Sanmiya, Kazutsuka
AU - Osamu, Ueno
AU - Matsuoka, Makoto
AU - Yamamoto, Naoki
PY - 1999/1/1
Y1 - 1999/1/1
N2 - The subcellular localization of plant farnesyl diphosphate synthase (FPPS) was examined. Immunocytochemical staining using anti-FPPS1 antibody followed by electron microscopy showed that FPPS1 was localized to chloroplasts of rice mesophyll cells. Subcellular fractions from wheat leaves were examined by immunoblot analysis. FPPS was detected in the chloroplast fraction in wheat, and was protected from proteolysis following trypsin treatment of chloroplasts. FPPS was also detected in the chloroplast fraction of a dicot plant, tobacco.
AB - The subcellular localization of plant farnesyl diphosphate synthase (FPPS) was examined. Immunocytochemical staining using anti-FPPS1 antibody followed by electron microscopy showed that FPPS1 was localized to chloroplasts of rice mesophyll cells. Subcellular fractions from wheat leaves were examined by immunoblot analysis. FPPS was detected in the chloroplast fraction in wheat, and was protected from proteolysis following trypsin treatment of chloroplasts. FPPS was also detected in the chloroplast fraction of a dicot plant, tobacco.
UR - http://www.scopus.com/inward/record.url?scp=0033103646&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033103646&partnerID=8YFLogxK
U2 - 10.1093/oxfordjournals.pcp.a029549
DO - 10.1093/oxfordjournals.pcp.a029549
M3 - Article
C2 - 10353221
AN - SCOPUS:0033103646
VL - 40
SP - 348
EP - 354
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 3
ER -