Localization of farnesyl diphosphate synthase in chloroplasts

Kazutsuka Sanmiya, Ueno Osamu, Makoto Matsuoka, Naoki Yamamoto

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The subcellular localization of plant farnesyl diphosphate synthase (FPPS) was examined. Immunocytochemical staining using anti-FPPS1 antibody followed by electron microscopy showed that FPPS1 was localized to chloroplasts of rice mesophyll cells. Subcellular fractions from wheat leaves were examined by immunoblot analysis. FPPS was detected in the chloroplast fraction in wheat, and was protected from proteolysis following trypsin treatment of chloroplasts. FPPS was also detected in the chloroplast fraction of a dicot plant, tobacco.

Original languageEnglish
Pages (from-to)348-354
Number of pages7
JournalPlant and Cell Physiology
Volume40
Issue number3
DOIs
Publication statusPublished - Jan 1 1999

Fingerprint

Geranyltranstransferase
Chloroplasts
chloroplasts
Triticum
Mesophyll Cells
subcellular fractions
wheat
Subcellular Fractions
Magnoliopsida
proteolysis
mesophyll
Trypsin
trypsin
Proteolysis
Tobacco
Anti-Idiotypic Antibodies
electron microscopy
Electron Microscopy
tobacco
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Localization of farnesyl diphosphate synthase in chloroplasts. / Sanmiya, Kazutsuka; Osamu, Ueno; Matsuoka, Makoto; Yamamoto, Naoki.

In: Plant and Cell Physiology, Vol. 40, No. 3, 01.01.1999, p. 348-354.

Research output: Contribution to journalArticle

Sanmiya, Kazutsuka ; Osamu, Ueno ; Matsuoka, Makoto ; Yamamoto, Naoki. / Localization of farnesyl diphosphate synthase in chloroplasts. In: Plant and Cell Physiology. 1999 ; Vol. 40, No. 3. pp. 348-354.
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