TY - JOUR
T1 - Localization of farnesyl diphosphate synthase in chloroplasts
AU - Sanmiya, Kazutsuka
AU - Ueno, Osamu
AU - Matsuoka, Makoto
AU - Yamamoto, Naoki
N1 - Funding Information:
We wish to thank Dr. T. Sasaki of the Rice Genome Research Program for providing the rice EST clone, C52647. We are grateful to Dr. T. Koshiba, Dr. N. Aoki and Dr. N. Inagaki for their technical advice. This work was supported by the Special Coordination Fund for Promoting Science and Technology, Enhancement of Center-of-Excellence, to National Institute of Agrobiological Resources from the Science and Technology Agency of Japan, and in part by a fund from the Ministry of Agriculture, Forestry and Fisheries of Japan.
PY - 1999
Y1 - 1999
N2 - The subcellular localization of plant farnesyl diphosphate synthase (FPPS) was examined. Immunocytochemical staining using anti-FPPS1 antibody followed by electron microscopy showed that FPPS1 was localized to chloroplasts of rice mesophyll cells. Subcellular fractions from wheat leaves were examined by immunoblot analysis. FPPS was detected in the chloroplast fraction in wheat, and was protected from proteolysis following trypsin treatment of chloroplasts. FPPS was also detected in the chloroplast fraction of a dicot plant, tobacco.
AB - The subcellular localization of plant farnesyl diphosphate synthase (FPPS) was examined. Immunocytochemical staining using anti-FPPS1 antibody followed by electron microscopy showed that FPPS1 was localized to chloroplasts of rice mesophyll cells. Subcellular fractions from wheat leaves were examined by immunoblot analysis. FPPS was detected in the chloroplast fraction in wheat, and was protected from proteolysis following trypsin treatment of chloroplasts. FPPS was also detected in the chloroplast fraction of a dicot plant, tobacco.
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U2 - 10.1093/oxfordjournals.pcp.a029549
DO - 10.1093/oxfordjournals.pcp.a029549
M3 - Article
C2 - 10353221
AN - SCOPUS:0033103646
VL - 40
SP - 348
EP - 354
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 3
ER -