TY - JOUR
T1 - Localization of human interleukin 13 receptor in non-haematopoietic cells
AU - Akaiwa, Mina
AU - Yu, Bin
AU - Umeshita-Suyama, Ritsuko
AU - Terada, Nobuhisa
AU - Suto, Hajime
AU - Koga, Tetsuya
AU - Arima, Kazuhiko
AU - Matsushita, Sho
AU - Saito, Hirohisa
AU - Ogawa, Hideoki
AU - Furue, Masutaka
AU - Hamasaki, Naotaka
AU - Ohshima, Koichi
AU - Izuhara, Kenji
N1 - Funding Information:
This work was supported in part by a Research Grant for Immunology, Allergy and Organ Transplant from the Ministry of Health and Welfare of Japan; a grant-in-aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan; a grant from Kanae Foundation for Life and SocioMedical Science; and the Hokuriku Seiyaku Research Award in Allergy from the Japan Allergy Foundation.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2001/1/21
Y1 - 2001/1/21
N2 - Although the functional roles of interleukin (IL-)13 in haematopoietic cells are well investigated, those in non-haematopoietic cells remain to be addressed. IL-13 exerts its actions by binding to the IL-13 receptor (IL-13R) on target cells, which is composed of IL-13Rα1 and the IL-4 receptor a chain (IL-4Rα). However, there has been no study of localization of IL-13R in each tissue. To address this question, we generated monoclonal anti-IL-13Rα1 antibody, and performed immunohistochemistry using this antibody and anti-IL-4Rα antibody. Distribution of these two components was the same in all examined tissues. Staining was positive in keratinocytes, hair follicles, and sebaceous and sweat glands in skin; in ciliated respiratory epithelial cells in nasal tissue; in heart muscle cells; in foveola cells, gastric glands, and the smooth muscle layer in stomach; and in hepatocytes in liver. However, staining was undetectable in brain and bone marrow. Fibroblasts and endothelial cells were stained in some tissues. These results provide clues to elucidate the known pathological roles of IL-13 in atopic dermatitis and allergic rhinitis, as well as its unknown physiological roles.
AB - Although the functional roles of interleukin (IL-)13 in haematopoietic cells are well investigated, those in non-haematopoietic cells remain to be addressed. IL-13 exerts its actions by binding to the IL-13 receptor (IL-13R) on target cells, which is composed of IL-13Rα1 and the IL-4 receptor a chain (IL-4Rα). However, there has been no study of localization of IL-13R in each tissue. To address this question, we generated monoclonal anti-IL-13Rα1 antibody, and performed immunohistochemistry using this antibody and anti-IL-4Rα antibody. Distribution of these two components was the same in all examined tissues. Staining was positive in keratinocytes, hair follicles, and sebaceous and sweat glands in skin; in ciliated respiratory epithelial cells in nasal tissue; in heart muscle cells; in foveola cells, gastric glands, and the smooth muscle layer in stomach; and in hepatocytes in liver. However, staining was undetectable in brain and bone marrow. Fibroblasts and endothelial cells were stained in some tissues. These results provide clues to elucidate the known pathological roles of IL-13 in atopic dermatitis and allergic rhinitis, as well as its unknown physiological roles.
UR - http://www.scopus.com/inward/record.url?scp=0035924916&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035924916&partnerID=8YFLogxK
U2 - 10.1006/cyto.2000.0814
DO - 10.1006/cyto.2000.0814
M3 - Article
C2 - 11145846
AN - SCOPUS:0035924916
VL - 13
SP - 75
EP - 84
JO - Cytokine
JF - Cytokine
SN - 1043-4666
IS - 2
ER -